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pGP-CMV-GCaMP6m

Plasmid

#40754

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6m

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDawn

Plasmid

#43796

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLPCX-VEcadTL

Plasmid

#45849

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

VE-cadherin (Cdh5 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-beta-catenin deltaN90

Plasmid

#36985

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

beta-catenin deltaN90 (CTNNB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90 (Constitutively Acti…

Promoter

EF1a

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pDD120

Plasmid

#119879

Purpose

Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_Che9c60 and Che9c61

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-9xGRE

Plasmid

#74736

Purpose

GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

Depositor

Luisa Dalla Valle

Article

Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

Insert

EGFP

Use

Zebrafish expression

Promoter

nine GC-responsive elements (9xGRE) from the rat …

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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Human SERCA2a (pcDNA3.1+)

Plasmid

#75187

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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rAAV2/cMG

Plasmid

#184539

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A589D; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTARA

Plasmid

#31491

Depositor

Kathleen Matthews

Article

Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

Insert

RNA polymerase

Expression

Bacterial

Mutation

N823D

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Michalis Averof, Charalambos Savakis

Article

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Promoter

anhydrotetracycline Ptet promoter

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mNaChBac-T2A-tdTomato

Plasmid

#60650

Purpose

Expresses mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBac-T2A-tdTomato

Expression

Mammalian

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Puro-SMAR)

Plasmid

#80391

Purpose

expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Puromycine

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mAIM2-Flag

Plasmid

#51537

Purpose

Express Flag-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-EGFPKASH

Plasmid

#124882

Purpose

labelling of neuronal nuclear envelope with EGFP

Depositor

Vassilis Pachnis

Article

Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

Insert

EGFP

Use

AAV

Tags

KASH

Promoter

CaMKII

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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