We narrowed to 9,400 results for: Coli

1. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. D. vulgaris Cas5c/pHMGWA

   Plasmid

   #81182

   Purpose

   Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas5c
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. D. vulgaris Cas8c/pHMGWA

   Plasmid

   #81183

   Purpose

   Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas8c
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E-Dam-myc no-pA

   Plasmid

   #82600

   Purpose

   3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   Dam-Myc (no pA)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSM14

   Plasmid

   #101645

   Purpose

   Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   efasCFP
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. U_(+36GFP)-Cre.ape

   Plasmid

   #71749

   Purpose

   U+36GFP-Cre fusion expression in E. Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   U_(pos36GFP)-Cre
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHL 651

   Plasmid

   #53016

   Purpose

   LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Inserts

   LacIq
   tetR
   

   Use

   Synthetic Biology

   Tags

   RBS(st3) and T1 terminator

   Expression

   Bacterial

   Mutation

   Promoter

   pCon

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. LovTAP N538E

   Plasmid

   #61431

   Purpose

   Expresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.

   Depositor

   Tobin Sosnick

   Article

   Strickland et al Nat Methods. 2010 Aug;7(8):623-6. doi: 1

   Insert

   LovTAP N538E
   

   Use

   Tags

   CBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac operator

   Available Since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pTL-4

   Plasmid

   #52033

   Purpose

   Inducible expression of GST-unc43 in E. coli

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   Unc-43 (unc-43 Nematode)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET-DEST42-SAR1-V5-His6

    Plasmid

    #67430

    Purpose

    Expression of hSar1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEN_TTmiRc2_BirA

    Plasmid

    #136521

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available Since

    June 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTEI128

    Plasmid

    #126209

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MolBC

    Plasmid

    #92210

    Purpose

    Expression of H. influenzae MolBC (ABC Transporter) in E. coli

    Depositor

    Douglas Rees

    Article

    Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

    Inserts

    MolC (HI1471 )
    MolB (HI1470 )
    

    Use

    Tags

    8x Histidine Tag and Enterokinase Cleavage Site b…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T1 human APC-B,C (1133–1189)

    Plasmid

    #132630

    Purpose

    expresses human APC-B,C (1133–1189) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

    Insert

    APC-B,C (1133–1189)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSM32

    Plasmid

    #101634

    Purpose

    Encodes the fluorescent protein Rosmarinus for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    Rosmarinus
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pThom77.2

    Plasmid

    #101646

    Purpose

    Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    amFP486
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST14-ARL5B

    Plasmid

    #67379

    Purpose

    Expression of native hArl5b in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL5B (ARL5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGS1044

    Plasmid

    #63797

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

    Insert

    3xFLAG followed by KlLEU2
    

    Use

    E. coli cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28(a)-p2c-eGFP

    Plasmid

    #242758

    Purpose

    Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli

    Depositor

    Jason Rasgon

    Article

    Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

    Insert

    P2C-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MBP-Streptavidin-RGG

    Plasmid

    #242451

    Purpose

    Expresses the MBP-Streptavidin-RGG fusion protein in E Coli.

    Depositor

    Benjamin Schuster

    Article

    Kelley et al Nat Commun. 2025 Apr 14;16(1):3521. doi:

    Insert

    MBP-Streptavidin-RGG (using LAF-1 RGG domain)
    

    Use

    Tags

    MBP and Steptavidin

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pProUSER13F4C

    Plasmid

    #178023

    Purpose

    E. coli - B. subtilis nicking vector pProUSER13F4C. Ap-BBR1; ΔsigF-insertion-site; Km; manR-PmanA

    Depositor

    Sheila Jensen

    Article

    Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

    Insert

    nicking site to be replaced with your gene of interest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pProUSER13E3F

    Plasmid

    #178026

    Purpose

    E. coli - B. subtilis nicking vector pProUSER13E3F. Ap-BBR1; yhgE-insertion-site; Spc; tetR-Ptet

    Depositor

    Sheila Jensen

    Article

    Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

    Insert

    nicking site to be replaced with your gene of interest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pProUSER13G2F

    Plasmid

    #178027

    Purpose

    E. coli - B. subtilis nicking vector pProUSER13G2F . Ap-BBR1; rsbP-insertion-site; Ery; tetR-Ptet

    Depositor

    Sheila Jensen

    Article

    Falkenberg et al ACS Synth Biol. 2021 Dec 17;10(12):3278-

    Insert

    nicking site to be replaced with your gene of interest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHInt-AppA-KanR-loxP

    Plasmid

    #232910

    Purpose

    Plasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    AppA (appA )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Pj5

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOPINF AtMKK5

    Plasmid

    #228396

    Purpose

    Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    MKK5 (MKK5 Mustard Weed)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-NFLtail

    Plasmid

    #232055

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFL tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Light tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET29_anti-SARS-CoV-2-NM1226

    Plasmid

    #209422

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1226
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pET29_anti-SARS-CoV-2-NM1230

    Plasmid

    #209423

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1230
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pET29_anti-SARS-CoV-2-Nanobody-C5

    Plasmid

    #209420

    Purpose

    Express anti-SARS-CoV-2-Nanobody C5 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-C5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET29_anti-SARS-CoV-2-Nanobody-F2

    Plasmid

    #209421

    Purpose

    Express anti-SARS-CoV-2-Nanobody F2 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-F2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge