We narrowed to 20,449 results for: Cre

Showing: 6601 - 6630 of 20449 results

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SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha

Plasmid

#179713

Purpose

Lentiviral expression of mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt11-V5

Plasmid

#35941

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb Wnt3 promoter-EGFP

Plasmid

#203792

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

EGFP

Use

Zebrafish expression

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-RpS12

Plasmid

#71504

Purpose

flySTARR-seq screening vector with RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT4

Plasmid

#183294

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT4

Use

Lentiviral

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT3

Plasmid

#183293

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CSF1R

Plasmid

#183277

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF1R gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF1R

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9B

Plasmid

#35919

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Expression

Mammalian

Mutation

SNP Met106-->Thr

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-rab5c-DN

Plasmid

#109737

Purpose

Multisite gateway vector for 3' tagging with dominant negative rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_JAK2

Plasmid

#183303

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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Tt-NylC_pET28a

Plasmid

#215431

Purpose

Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Tt-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC15-P1B3

Plasmid

#202856

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC15

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC11-P1A11

Plasmid

#202852

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC11

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMM585-Tier1-PhCMV-Igk

Plasmid

#169486

Purpose

Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Igk secretion signal (IGK Human)

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-PLK1

Plasmid

#46312

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
PLK1 DNA-1
linker
PLK1 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pV2A-T

Plasmid

#153022

Purpose

Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

Depositor

Vito Valiante

Article

Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pOCC114

Plasmid

#118904

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with TEV protease, two glycines

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pT3TS-Dest_R1-R3

Plasmid

#140878

Purpose

166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-Foxd3 in situ

Plasmid

#37098

Depositor

Patricia Labosky

Article

Labosky et al Mech Dev. 1998 Aug;76(1-2):185-90.

Insert

Foxd3 (Foxd3 Mouse)

Use

In situ hybridization probe

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-Rab8b in pCSDEST2

Plasmid

#140881

Purpose

Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8b (rab8b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt2-V5

Plasmid

#35925

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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JC101: pMVP/Ad/PK-DEST

Plasmid

#121845

Purpose

pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_91

Plasmid

#130539

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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