We narrowed to 21,470 results for: SPR
-
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
TagsMBPExpressionBacterialMutationD832APromoterT7Available SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…ExpressionMammalianPromoterCAGAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDP025
Plasmid#103874PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactisDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
UseCRISPRExpressionYeastPromoterScTDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180268PurposeLentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNADepositorInsertGARP sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgOprk1
Plasmid#159903PurposeMutagenesis of Oprk1DepositorInsertOprk1 (Oprk1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROS10
Plasmid#107924PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
MLKL gRNA (BRDN0001148608)
Plasmid#77539Purpose3rd generation lentiviral gRNA plasmid targeting human MLKLDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TetO-dCas9-mD3A
Plasmid#78255PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterCMVAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x6
Plasmid#58776PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD19_STARR-seq_plants
Plasmid#117379PurposeSTARR-seq vector with 35s core promoter for transient expression in plant protoplastsDepositorTypeEmpty backboneTagsGFP derived from pMDC107ExpressionPlantPromoter35s core promoterAvailable SinceNov. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMZ376
Plasmid#74213PurposeCombined sgRNA/Cas9 vector with KanMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
PINK1 gRNA (BRDN0001145357)
Plasmid#78036Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…PromoterxylR-driven promoterAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL-5LTR-GW-A
Plasmid#138480PurposeFor expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.DepositorInsertGateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AmCyan
Plasmid#138481PurposeFor cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.DepositorInsertAmCyan
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK9 gRNA (BRDN0001146478)
Plasmid#77169Purpose3rd generation lentiviral gRNA plasmid targeting human CDK9DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK11 gRNA (BRDN0001146880)
Plasmid#75912Purpose3rd generation lentiviral gRNA plasmid targeting human STK11DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only