We narrowed to 10,196 results for: otos

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Isl1

Plasmid

#32929

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Isl1 (Isl1 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-Ngn2

Plasmid

#32928

Purpose

Retroviral expression of mouse Ngn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Ngn2 (Neurog2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pES005 (pTet-qacR wt/pQacA-deGFP)

Plasmid

#69034

Purpose

reporter with wild-type qacR on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mApple-Annexin(A4)-12

Plasmid

#54867

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Annexin (A4) (ANXA4 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

Plasmid

#199351

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80R

Plasmid

#118350

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to R.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-YR-ICUE-mGold2t

Plasmid

#231790

Purpose

Visualization of cAMP dynamics using YR-ICUE-mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

YR-ICUE-mGold2t

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_EmGFP

Plasmid

#113892

Purpose

Mammalian (inducible) expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

Emerald GFP

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pB[U6-3 wex2 PUb-DsRed]

Plasmid

#169010

Purpose

For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

white ex2 sgRNA

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster U6:3

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-2xNLS-mTurquoise2

Plasmid

#118025

Purpose

An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Promoter

hSyn1

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Insect

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
PDCD1-DEM (P1#5)

Plasmid

#227738

Purpose

Designer Epigenome Modifier (DEM) designed to target the PDCD1-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting PDCD1-promoter

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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LAG3-DEM (L3#1)

Plasmid

#227739

Purpose

Designer Epigenome Modifier (DEM) designed to target the LAG3-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting LAG3-promoter (Homo sapiens)

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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3xDBE_RE::Renilla

Plasmid

#124535

Purpose

Foxo Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

FOXO Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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cpLID-micro

Plasmid

#168995

Purpose

circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

mCh-sspB-T2A-cpLID-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano15/pBig

Plasmid

#51962

Purpose

Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPERIOR.puro-shp53

Plasmid

#38035

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shp53 (TP53 Human)

Use

RNAi

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only