We narrowed to 7,509 results for: PAC;

1. pBluescript-IC3-FLAG-Tev-ZZ

   Plasmid

   #170311

   Purpose

   Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Outer Dynein Arm Intermediate Chain 3
   

   Use

   Tags

   FLAG, ZZ

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459V2.0-SpCas9-HF1

   Plasmid

   #108293

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-FLAG-Dicer-delPAZ

   Plasmid

   #51367

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to PAZ domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-FLAG-Dicer-delDEAD

   Plasmid

   #51364

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to DEAD domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-FLAG-Dicer-delDUF

   Plasmid

   #51365

   Depositor

   Narry Kim

   Article

   Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   From N terminus to DUF domain deletion

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet-15b-microID

   Plasmid

   #172881

   Purpose

   expresses microID in E. coli

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   microID
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   OsU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1_VNp6-DARPinOFF7

   Plasmid

   #182398

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-DARPinOFF7
   

   Use

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG201 HIPK1 3'UTR

   Plasmid

   #12012

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   HIPK1 3'UTR (HIPK1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99679

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG145 HIPK1 3'UTR

    Plasmid

    #12003

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N10 3'UTR (HIPK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Burden Monitor phi80 version

    Plasmid

    #66074

    Purpose

    The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Use

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pV92.2

    Plasmid

    #78409

    Purpose

    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct

    Depositor

    Andrew Fire

    Article

    Silas et al Science. 2016 Feb 26;351(6276):aad4234.

    Inserts

    RT-Cas1
    Cas2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEYFP-Sec24D

    Plasmid

    #66614

    Purpose

    YFP fusion of Sec24D

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24D (SEC24D Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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