59,541 results

1. GFP-UBR5

   Plasmid

   #52050

   Purpose

   CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

   Depositor

   Darren Saunders

   Article

   Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

   Insert

   UBR5 (UBR5 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GPR87-DuET

   Plasmid

   #213293

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR87 (GPR87 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TOM20*-SBP-GFP

   Plasmid

   #120173

   Purpose

   Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
   

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Mutation

   Truncated TOM20: 1-30 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2207

   Plasmid

   #143354

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB3L2 (CREB3L2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST17-UbE2G1

   Plasmid

   #15790

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

6. mCherry KNL1 wt hardened

   Plasmid

   #45224

   Depositor

   Iain Cheeseman, Michael Lampson

   Article

   Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

   Insert

   mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
   

   Tags

   TEV S15

   Expression

   Mammalian

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. YFP-NOP56-C3

   Plasmid

   #134536

   Purpose

   Expresses YFP-NOP56 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   NOP56 (NOP56 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1-eTLR

   Plasmid

   #214737

   Purpose

   encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   AAVS1-eTLR (PPP1R12C Synthetic, Human, CMV, Anguilla japonica, influenza, Bovine, Rabbit, Chicken)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-HA-Flag-natT1R2

   Plasmid

   #113944

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-MAP1LC3B

    Plasmid

    #137757

    Purpose

    Expression of LC3B

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid

    #181974

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TLCV2-LoxP

    Plasmid

    #127098

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system.

    Depositor

    Adam Karpf

    Insert

    Cas9-2A-eGFP
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

    Plasmid

    #157396

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    HLA-E (HLA-E Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-AIF

    Plasmid

    #16182

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Apoptosis-inducing factor (AIFM1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-cT3G-cERP2-EGR1

    Plasmid

    #192913

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    EGR1 (EGR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLHKI-pGFPN3

    Plasmid

    #21917

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKI in pGFP-N3 (HK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is full length human HKI in pGFP-N3

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF3054

    Plasmid

    #144530

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SALL4 (SALL4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge