We narrowed to 11,278 results for: VARS

1. pLKO-Cre sgAmotl2 v3

   Plasmid

   #158602

   Purpose

   lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Amotl2 sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. VEGF binding scFv G6

   Plasmid

   #111716

   Purpose

   Yeast Surface Display of G6 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6
   

   Tags

   cmyc tag

   Expression

   Yeast

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. dxCas(3.6)-VPR

   Plasmid

   #108388

   Purpose

   Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.6)-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTracer_Cr-TRP1

   Plasmid

   #64881

   Purpose

   Expresses CrTRP1 in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

   Insert

   Transient receptor potential 1
   

   Tags

   eGFP

   Promoter

   CMV

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Sniper-Cas9

   Plasmid

   #138559

   Purpose

   Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   Sniper-Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-TIE-V5/HIS

   Plasmid

   #205612

   Purpose

   Expression of human TIE receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TIE receptor tyrosine kinase (TIE1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AM77_pU6.Sa-gRNA.CLYBL

   Plasmid

   #199237

   Purpose

   Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. aureus gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE80L-SH3

   Plasmid

   #160331

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   SH3
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CABE-T2.19

    Plasmid

    #193265

    Purpose

    Expression of CABE-T2.19 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T2.19
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS1988

    Plasmid

    #49119

    Purpose

    AAV plasmid with CAGGS promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-CAGGS-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Expression

    Mammalian

    Mutation

    R203K

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. DEL-pHG165a

    Plasmid

    #90064

    Purpose

    LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    DEL
    

    Expression

    Bacterial

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS_375

    Plasmid

    #182132

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_SKAP2-1/1

    Plasmid

    #91447

    Purpose

    Protein expression and purification of human SH3 domain construct SKAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SKAP2-1/1 (SKAP2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LacI-11/109-pHG165c

    Plasmid

    #90059

    Purpose

    Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LacI-11/109
    

    Expression

    Bacterial

    Mutation

    109

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV iGluu

    Plasmid

    #105832

    Purpose

    Fast indicator for extracellular glutamate imaging

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    CMV

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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