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We narrowed to 18,247 results for: multi

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Showing: 4421 - 4440 of 18247 results

  1. pJC8

    Plasmid
    #170757
    Purpose
    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.
    Depositor
    Roberto Dominguez
    Publication
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Tags
    site 1- V5 tag and site 2- FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170757-plasmid-map-sequence-id-334720

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  4. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Publication
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  5. pPV477

    Plasmid
    #42930
    Depositor
    James Lok
    Insert
    attL1::egfp (870 bp)::attL2
    Use
    Multi-site gateway donor plasmid
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42930-plasmid-map-sequence-id-58193

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  9. cfmTurquoise-N

    Plasmid
    #139284
    Purpose
    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
    Depositor
    Ikuo Wada
    Publication
    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cfmTurquoise
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    139284-plasmid-map-sequence-id-291944

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  14. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tagBFP
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177824-plasmid-map-sequence-id-348950

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  16. pMax_mOrange_G299C

    Plasmid
    #177829
    Purpose
    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Publication
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mOrange_G299C
    Expression
    Mammalian
    Mutation
    G299C
    Promoter
    CMV
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    177829-plasmid-map-sequence-id-348954

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  20. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60620-plasmid-map-sequence-id-99574

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Showing: 4421 - 4440 of 18247 results