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pcDNA3.1-Hpep1-betaArrestin2-T2A-mTagBFP2

Plasmid

#194680

Purpose

CMV driven co-expression of peptide complementing GPCR recorder (beta-arrestin 2 fusion) and mTagBFP2

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Hpep1-betaArrestin2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10873

Plasmid

#183961

Purpose

U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

Plasmid

#172508

Purpose

Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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TurboID-EV

Plasmid

#215085

Purpose

Expresses TurboID in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-TPD54

Plasmid

#172454

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Tags

GFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 IRES Cherry

Plasmid

#232109

Purpose

Lentiviral expression of human INTS4

Depositor

Tom Misteli

Article

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Tags

IRES CHERRY

Expression

Mammalian

Mutation

Wild Type

Promoter

EF 1alpha

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-FOXO3a WT

Plasmid

#8360

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

FOXO3a (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1

Plasmid

#53159

Purpose

Expresses GST-HA tagged human MEK1 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-Lck-mCherry-ibARK-4x6T

Plasmid

#241243

Purpose

Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

DIO-Lck-mCherry-ibARK-4x6T

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-iLACCO1.2

Plasmid

#208101

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

Plasmid

#240799

Purpose

CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

mNeonGreen-mCherry-CAAX

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1-E209A

Plasmid

#141155

Purpose

Mammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysis

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

MFN 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

changed Glutamic acid at position 209 to Alanine …

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-R-iLACCO1.1

Plasmid

#208103

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_Cre

Plasmid

#201198

Purpose

AAV vector for cre expression under EF1 promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

cre
EF1alpha promoter

Use

AAV

Expression

Bacterial and Mammalian

Mutation

nuclear targeting cre

Promoter

N/A and hEF1alpha promoter

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 WT

Plasmid

#41901

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_mGL_PP7

Plasmid

#232440

Purpose

expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

mGreenLantern

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1950

Plasmid

#205983

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Promoter

T7 (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203229

Purpose

Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Cre-dependent, membrane-localized Optopatch

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ10878

Plasmid

#183964

Purpose

CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D156R, E292R, D235R, D350R

Promoter

CAG

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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