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eGFP-KCC2

Plasmid

#104075

Purpose

expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

Depositor

Igor Medyna

Article

Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRVdG-4Cre

Plasmid

#98034

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

mCre

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mGM-CSF

Plasmid

#74465

Purpose

expression of mGM-CSF in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/cyto-iAspSnFR

Plasmid

#201394

Purpose

CMV overexpression of cyto-iAspSnFR in mammalian cell lines

Depositor

Kai Johnsson

Article

Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

Insert

cyto-iAspSnFR

Expression

Mammalian

Mutation

gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

Promoter

CMV

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-VP64-dCas9-VP64

Plasmid

#232179

Purpose

Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

VP64-dCas9-VP64

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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aMHC-mCherry-Rex-Blasticidin

Plasmid

#21228

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

mCherry

Available Since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-Flag

Plasmid

#180407

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV/eGFP-U6-sgRNA

Plasmid

#170544

Purpose

A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-CXCR4

Plasmid

#98944

Purpose

Mammalian expression plasmid for untagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-CCR5

Plasmid

#98950

Purpose

Mammalian expression plasmid for N-terminal HA-tagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

HA (YPYDVPDYA) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-R-diLACCO1

Plasmid

#208105

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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CR1-isoformB-CD4d3+4-bio

Plasmid

#73099

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CR1-isoformB with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CR1 isoform B (CR1 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM2

Plasmid

#82537

Purpose

Lentiviral vector for expressing HRP-LRRTM2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM2 (LRRTM2 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD3

Plasmid

#195841

Purpose

Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD3 (FZD3 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

I292V and V495I

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-HALO-CTERM-GFP

Plasmid

#185777

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CD47-CD4d3+4-bio

Plasmid

#73121

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD47 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD47 antigen (CD47 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AchLightG

Plasmid

#187464

Purpose

Expresses green acetylcholine indicator AchLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightG

Use

AAV

Tags

Igκ leader

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-HALO-NTERM-GFP

Plasmid

#185772

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Tuba

Plasmid

#22214

Depositor

Pietro De Camilli

Article

Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

Insert

Tuba (DNMBP Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only