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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 39,129 results for: Eras

Showing: 6661 - 6690 of 39129 results

  2. pGBW-m4046955

    Plasmid
    #145616
    Purpose
    Bacterial Expression plasmid for RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145616-plasmid-map-sequence-id-307396

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  7. pCDM4-GLY

    Plasmid
    #49808
    Purpose
    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49808-plasmid-map-sequence-id-74623

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  8. pGBW-m4046809

    Plasmid
    #145685
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145685-plasmid-map-sequence-id-297845

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  12. pGBW-m4046795

    Plasmid
    #145598
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145598-plasmid-map-sequence-id-297835

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  13. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

    Plasmid
    #216385
    Purpose
    Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Norrin (NDP Human)
    Use
    Baculovirus
    Tags
    GP64 signal sequence-MBP-3C and Rho1D4
    Expression
    Insect
    Mutation
    C93A/C95A/C131A
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216385-plasmid-map-sequence-id-433265

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  14. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

    Plasmid
    #214459
    Purpose
    Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214459-plasmid-map-sequence-id-423212

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  26. pTJV1Sc2-tetA

    Plasmid
    #166982
    Purpose
    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    CspRecT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166982-plasmid-map-sequence-id-331335

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  28. plentiCRISPR-sgGOT1

    Plasmid
    #72874
    Purpose
    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    sgGOT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. pFPL-Cg

    Plasmid
    #61652
    Purpose
    Gateway vector for generating cyan fluorescent protein fusions in fungi
    Depositor
    Mark Farman
    Article
    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.
    Insert
    enhanced cyan fluorescent protein
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6661 - 6690 of 39129 results