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Showing: 11101 - 11150 of 11588 results

  1. AAV-hSyn-ZipT-2A-IvfChr (citrine, KV2.1)

    Plasmid
    #221619
    Purpose
    A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.
    Depositor
    John Lin
    Article
    Mermet-Joret et al eLife 2023 12:RP90327
    Insert
    ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
    Use
    AAV
    Mutation
    ZipACR (I151T) with soma targeting, vfChrimson w…
    Promoter
    Human Synapsin
    Available Since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    221619-plasmid-map-sequence-id-442289

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  2. AAV-hSyn-ZipT-2A-IvfChr (mCherry, KV2.1)

    Plasmid
    #221621
    Purpose
    A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-mCHerry with soma targeting KV2.1 motif.
    Depositor
    John Lin
    Article
    Mermet-Joret et al eLife 2023 12:RP90327
    Insert
    ZipACR (I151V)KV2.1-2A-IvfChr-mCherry-Kv2.1
    Use
    AAV
    Mutation
    ZipACR (I151T) with soma targeting, vfChrimson w…
    Promoter
    Human Synapsin
    Available Since
    Aug. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221621-plasmid-map-sequence-id-442207

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  3. AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)

    Plasmid
    #221618
    Purpose
    A recombinant AAV2 plasmid encoding the ZipACR I151V mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.
    Depositor
    John Lin
    Article
    Mermet-Joret et al eLife 2023 12:RP90327
    Insert
    ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
    Use
    AAV
    Mutation
    ZipACR (I151V) with soma targeting, vfChrimson w…
    Promoter
    Human Synapsin
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221618-plasmid-map-sequence-id-441799

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  4. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  5. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Article
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

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  6. AA324

    Plasmid
    #215953
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215953-plasmid-map-sequence-id-431481

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  9. p Pcrg1: Khd4-Ada-Gfp (CbxR)

    Plasmid
    #203734
    Purpose
    Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, fol
    Depositor
    Michael Feldbrügge
    Article
    Sankaranarayanan et al Proc Natl Acad Sci U S A. 2023 Aug 22;12
    Insert
    Khd4-Ada-Gfp
    Tags
    Gfp
    Expression
    Bacterial
    Promoter
    Pcrg1
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203734-plasmid-map-sequence-id-404932

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  26. pENT-L1-Kozak-Ark-L2

    Plasmid
    #186362
    Purpose
    Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Ciona robusta Aurora kinase A
    Use
    Expression of a fluorescent centrosome marker
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186362-plasmid-map-sequence-id-373433

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  27. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

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  37. pMta2-EosHalo-TAFiP

    Plasmid
    #140959
    Purpose
    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    metastasis-associated gene family, member 2 (Mta2 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140959-plasmid-map-sequence-id-338415

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  39. C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

    Plasmid
    #162950
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
    Use
    Synthetic Biology
    Available Since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    162950-plasmid-map-sequence-id-331914

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Showing: 11101 - 11150 of 11588 results