We narrowed to 4,564 results for: HRE
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Plasmid#120513PurposeBarcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Thr58Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Threonine to Alanine at a…PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-AausFP1
Plasmid#191096PurposeTo express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-AausFP1
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV enhancer and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dMFot
Plasmid#44558DepositorInsertsPTETREG promoter
rtetR-M2::FFF
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationCompared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…PromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-LAP-KNL1-M3
Plasmid#115896PurposeUsed for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertKNL1-M3 (KNL1 Human)
UseTagsEYFPExpressionMammalianMutationFragment of KNL1 containing three KNL1 repeat seq…PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
BE3-NLS-NLS-P2A-BLAST
Plasmid#135348PurposeExpression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertBE3-2XNLS
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain
Plasmid#86020PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged alanine 82 to valine, threonine 230 to al…PromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86023PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationLacks mucin-like domain: Changed threonine 230 to…PromoterCMV IEAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86025PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationLacks mucin-like domain; Changed alanine 82 to va…PromoterCMV IEAvailable sinceJuly 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G
Plasmid#233063PurposeExpresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.DepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsALFA and HiBiTExpressionMammalianMutationThreonine 923 to GlycinePromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-SS-VN155(I152L)-IRE1-dLD
Plasmid#217752PurposeExpresses the VN fragment of BiFC-IRE1-dLDDepositorInsertSerine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
UseTagsVenus (1-154, I152L)ExpressionMammalianMutationdeleted amino acids 29-408PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC131: pAAV.CMV-CasRx-VEGFA sgRNA array
Plasmid#203444PurposePlasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAsDepositorInsertsU6-VEGFA sgRNA array
RfxCas13d
UseAAVTagsHA and NLSExpressionMammalianMutationPromoterCMV and U6Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA
Plasmid#177342PurposeAAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequenceDepositorInsertdead hSaCas9
UseAAV and CRISPRTags3x GCN4 peptide, 3xHA, and NLSExpressionMammalianMutationD10A, N580APromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T
Plasmid#147590PurposeInsect Expression of DmThor-CG32016-EDepositorInsertDmThor-CG32016-E (Thor Fly)
UseTagsExpressionInsectMutationDmThor two silent mutations compared to the seque…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC
Plasmid#148427PurposeMammalian Expression of HsAGO2-K663AL697YDepositorInsertHsAGO2-K663AL697Y (AGO2 Human)
UseTagsExpressionMammalianMutationthree silent mutations and the sequence differs i…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC
Plasmid#148428PurposeMammalian Expression of HsAGO2-P593GA623FK663AL697YDepositorInsertHsAGO2-P593GA623FK663AL697Y (AGO2 Human)
UseTagsExpressionMammalianMutationthree silent mutations and the sequence differs i…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, T805C, C813S)-Strep
Plasmid#169749PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Threonine 805 to Cysteine…PromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-R21E_B
Plasmid#145997PurposeInsect Expression of DmTral-R21EDepositorInsertDmTral-R21E (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-R42E_B
Plasmid#145998PurposeInsect Expression of DmTral-R42EDepositorInsertDmTral-R42E (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-E23K_B
Plasmid#145989PurposeInsect Expression of DmTral-E23KDepositorInsertDmTral-E23K (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral_99-393-V5His6_D
Plasmid#146137PurposeInsect Expression of DmTral_99-393DepositorInsertDmTral_99-393 (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only