We narrowed to 7,397 results for: Lif

1. pBW101ParsR-gfp

   Plasmid

   #78636

   Purpose

   pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   J101-rbs32-arsR-t-ParsR-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCSIIpuro2-H2B-iRFP

   Plasmid

   #197352

   Purpose

   Encoding nuclear-targeted iRFP713.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   iRFP with nucleus-targeting sequence
   

   Use

   Lentiviral

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET251-pUC 12xMS2V6 Loxp KANr Loxp

   Plasmid

   #104392

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-EF1a_NKX2-1_Short_V5-GFP

   Plasmid

   #221497

   Purpose

   NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5-linker-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMA2640

   Plasmid

   #25434

   Purpose

   Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   reverse tetracycline-regulated transactivator Advanced
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScalps_Puro

   Plasmid

   #99636

   Purpose

   Empty backbone, 3rd generation lentiviral vector

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   SFFVp

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBbsr2-EKARrEV-NLS

    Plasmid

    #173855

    Purpose

    Encoding EKARrEV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCS2-mCherry-Rab11a

    Plasmid

    #184035

    Purpose

    Expresses mCherry Rab11a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

    Insert

    Rab11a
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-UBC_NKX2-1_Short_V5-GFP

    Plasmid

    #221499

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3789 pmko-neo RNAi-GFP

    Plasmid

    #8881

    Depositor

    Stanley Korsmeyer

    Article

    Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

    Insert

    GFP
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pWPI-TFDP1

    Plasmid

    #114299

    Purpose

    Constitutive expression of the TFDP1 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    TFDP1 (TFDP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

18. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSIIbsr-EKARrEV-NLS

    Plasmid

    #173854

    Purpose

    A lentiviral vector for EKARrEV-NLS

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rabenosyn-5

    Plasmid

    #37538

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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