We narrowed to 6,547 results for: proc

1. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ190

   Plasmid

   #162667

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SARS-CoV2 Nucleocapsid

   Plasmid

   #153201

   Purpose

   Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

   Depositor

   Peter Klein

   Article

   Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Use

   Tags

   Myc tag (5X)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ189

   Plasmid

   #162666

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

   Plasmid

   #171630

   Purpose

   Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

   Depositor

   Christopher A French, Chris French

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   BioTAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCJ202

   Plasmid

   #162675

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W159C, S238C; codon optimized for expression in E…

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE3G-GSX2-IRES2-EBF1

   Plasmid

   #96966

   Purpose

   Dox-inducible expression of Gsx2 and Ebf1 in mammalian cells

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GSX2 and EBF1 (bicistronic insert) (Gsx2, Ebf1 Mouse)
   

   Use

   Tags

   FLAG (on EBF1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

   Plasmid

   #177320

   Purpose

   Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).

   Depositor

   Xiaolin Nan, Steven Chu

   Article

   Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

   Insert

   KRAS 4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   FKBP-derived dimerization domain (DD, or DmrB) an…

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   TetOn-CMV

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCJ191

    Plasmid

    #162668

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEXP5-aHL_spytag_his

    Plasmid

    #107334

    Purpose

    Monomeric SpyTag-6×-His alpha-hemolysin

    Depositor

    George Church

    Article

    Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    alpha-hemolysin-SpyTag-6xHis
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid

    #162876

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein tagged with mEos2
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ206

    Plasmid

    #162679

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E226T; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. S10A mutant of H3S10ph biosensor

    Plasmid

    #120810

    Purpose

    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 10 is mutated to Alanine, and Threonine 3,…

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJ211

    Plasmid

    #162684

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaK2:G19; codon optimized for expression in E.…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCJ197

    Plasmid

    #162670

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S131G; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pABD1275

    Plasmid

    #68017

    Purpose

    PIF1 protein overproduction for purification from bacterial cells

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 Mustard Weed, AT2G20180)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Codon Optimized for bacterial expression (GenScri…

    Promoter

    T7

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-pBG-mCRE98-NLS-EGFP-WPRE

    Plasmid

    #239977

    Purpose

    adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

    Depositor

    Sinisa Hrvatin

    Article

    Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    EGFP (eGFP Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pBG + mCRE98

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPF101-pfQC-cyclase dead

    Plasmid

    #184878

    Purpose

    expresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutant

    Depositor

    Ferenc Scheeren

    Article

    Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    pfQC-cyclase dead
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    F77A & Q79A

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCJ205

    Plasmid

    #162676

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C224A, C529A; codon optimized for expression in E…

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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