We narrowed to 6,266 results for: wus

1. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97311

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiCRISPR v2-sgBAX-2

   Plasmid

   #210130

   Purpose

   knock out BAX in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Apoptosis regulator BAX (BAX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCRISPR v2-sgBAK-2

   Plasmid

   #210132

   Purpose

   knock out BAK in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Bcl-2 homologous antagonist/killer (BAK1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPR v2-sgBAK-1

   Plasmid

   #210131

   Purpose

   knock out BAK in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Bcl-2 homologous antagonist/killer (BAK1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti-Cas9-VQR-Blast

   Plasmid

   #87155

   Purpose

   lentiviral expression of SpCas9-VQR (NGA PAM restricted)

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   SpCas9-VQR(D1135V,R1335Q,T1337R)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D1135V,R1335Q,T1337R

   Promoter

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMThy1.1_hDDX5_FL_D248N

   Plasmid

   #88874

   Purpose

   constitutive expression of DDX5 D248N in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D248N

   Promoter

   MSCV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-dCas12a-A3B

   Plasmid

   #193642

   Purpose

   Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3B, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-dCas12a-A3G

   Plasmid

   #193643

   Purpose

   Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3G, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJM1-FH-AGO2-5xA

    Plasmid

    #91979

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-Puro-FH-AGO2-5xE

    Plasmid

    #91993

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLJM1-FH-AGO2-5xE

    Plasmid

    #91981

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97309

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.3-hALDOA D34S

    Plasmid

    #210810

    Purpose

    To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 34 of ALDOA mutated to serine

    Promoter

    CMV

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-Puro-FH-AGO2-5xA

    Plasmid

    #91992

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti6/V5-DEST-HOXA1

    Plasmid

    #31209

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HOXA1 (HOXA1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCMV5-Flag-Ago2-FDM3

    Plasmid

    #72210

    Purpose

    Mammalian expression of aa 227-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-226

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCMV5-Flag-Ago2-FDM1

    Plasmid

    #72208

    Purpose

    Mammalian expression of aa 1-372 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 373-860

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCMV5-Flag-Ago2-FDM2

    Plasmid

    #72209

    Purpose

    Mammalian expression of aa 1-517 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 518-860

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCMV5-Flag-Ago2-FDM4

    Plasmid

    #72211

    Purpose

    Mammalian expression of aa 373-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-372

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only