We narrowed to 11,766 results for: nsf

1. AAV-U6-sgRNA-EF1a-LibVec

   Plasmid

   #239591

   Purpose

   AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

   Depositor

   Andreas Moor

   Article

   Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti HsATP13A2 P652_E653 del

   Plasmid

   #213700

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

   Depositor

   Veerle Baekelandt

   Article

   Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

   Insert

   human ATP13A2 P652_E653 del (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   WT, D962N

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

   Plasmid

   #207135

   Purpose

   DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKG-GW1-hCIT

   Plasmid

   #203166

   Purpose

   Yeast expression vector for human DHDDS

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Insert

   DHDDS (DHDDS Human)
   

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-Gata6-3xT7

   Plasmid

   #200889

   Purpose

   Inducible lentiviral expression of Gata6

   Depositor

   Kai Ge

   Article

   Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

   Insert

   Gata6 (Gata6 Mouse)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   3xT7

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV10-3xFLAG-prs-hMIGA2 (170-593)

   Plasmid

   #192833

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   3xFLAG-PreScission cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mitoTALECD for_atp1_1397NC

   Plasmid

   #186204

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Promoter

   Arabidopsis RPS5A

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mitoTALECD for_-20G_1333CN

   Plasmid

   #186200

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mitoTALECD for_ -13G_1397CN

   Plasmid

   #186199

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mitoTALECD for_ -6G_1333CN

    Plasmid

    #186198

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid

    #182887

    Purpose

    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    silet mutations in aa 197 - 203 to confer resista…

    Promoter

    EF1alpha core

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid

    #182885

    Purpose

    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    stop codon introduced 2 amino acids downstream of…

    Promoter

    EF1alpha core

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-SopB(172-554)

    Plasmid

    #183664

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 172-554

    Promoter

    CMV

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-SopB(211-554)

    Plasmid

    #183665

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 211-554

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAID1.2-EF1a-NmClover3-hsLem2

    Plasmid

    #179524

    Purpose

    Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LEMD2 (LEMD2 Human)
    

    Tags

    mClover3-mAID

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR_P4-P1R:PER36modp

    Plasmid

    #128431

    Purpose

    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    PEROXISADE36 (AT3G50990 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Mutation

    Premature stop codons introduced into the Xero1 (…

    Promoter

    PEROXISADE36

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCri-H6-TEV-GRAB-STREP

    Plasmid

    #128499

    Purpose

    Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x) and Strep tag (2x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB61 - pL0_V2 (CDS1)

    Plasmid

    #123183

    Purpose

    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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