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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,191 results for: his

Showing: 8961 - 9000 of 22191 results

  1. pBAD-luxKOk

    Plasmid
    #229944
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.
    Insert
    luxKOk
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229944-plasmid-map-sequence-id-457161

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  2. pBAD-luxGold

    Plasmid
    #229943
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.
    Insert
    luxGold
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229943-plasmid-map-sequence-id-457160

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  3. pUB-MSP1D1

    Plasmid
    #161609
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1 ( Q138W, L181W, Y236W)
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161609-plasmid-map-sequence-id-317666

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  4. pBAF

    Plasmid
    #104152
    Purpose
    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.
    Depositor
    Robert Craigie
    Article
    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95
    Insert
    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    Use
    Tags
    N-terminal His tag with thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    104152-plasmid-map-sequence-id-201395

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  8. KRASB

    Plasmid
    #25153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KRASb:M1-K169 (KRAS Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Q61H
    Promoter
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pBTSG

    Plasmid
    #159420
    Purpose
    Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159420-plasmid-map-sequence-id-321535

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  15. pETSG

    Plasmid
    #159418
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159418-plasmid-map-sequence-id-321533

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  25. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Use
    Tags
    His and mRuby3
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

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Showing: 8961 - 9000 of 22191 results