Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 13,911 results for: NTS

Showing: 11201 - 11250 of 13911 results

  1. pOpen-BovDNTT

    Plasmid
    #165580
    Purpose
    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    Bovine DNTT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165580-plasmid-map-sequence-id-338558

    Enlarge

  2. mEmerald-Desmin-C-18

    Plasmid
    #54059
    Purpose
    Localization: Intermediate Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Desmin (DES Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54059-plasmid-map-sequence-id-261656

    Enlarge

  3. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    David Bartel
    Article
    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12178-plasmid-map-sequence-id-181181

    Enlarge

  5. pBOB-VE-cadherin-tdTomato

    Plasmid
    #118733
    Purpose
    To express VE-cadherin tagged with tdTomato on C-terminus
    Depositor
    John Cooper
    Insert
    Cadherin 5 (CD144) (CDH5 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Expression
    Mutation
    I503T (Common SNP-see comments)
    Promoter
    CMV
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118733-plasmid-map-sequence-id-229276

    Enlarge

  8. pBOB-Lck-tdTomato

    Plasmid
    #118739
    Purpose
    To express membrane targeted tdTomato
    Depositor
    John Cooper
    Insert
    Lck domain (LCK Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Expression
    Mutation
    H8N (see comments)
    Promoter
    CMV
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118739-plasmid-map-sequence-id-229130

    Enlarge

  9. mEmerald-Desmin-N-18

    Plasmid
    #54060
    Purpose
    Localization: Intermediate Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Desmin (DES Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. mEmerald-GFAP-N-18

    Plasmid
    #54107
    Purpose
    Localization: Intermediate Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    GFAP (GFAP Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. 4LG8

    Plasmid
    #60095
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRPF19:JMC069-A01:C226630 (PRPF19 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. CMV500 A-C/EBPβ

    Plasmid
    #33363
    Depositor
    Charles Vinson
    Insert
    Cebpb (Cebpb Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Dominant Negative (see comments)
    Promoter
    CMV
    Available since
    Dec. 14, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. PARP2A

    Plasmid
    #42450
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP2A (PARP2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. NUMBLA

    Plasmid
    #42420
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUMBLA (NUMBL Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pFGL821-8

    Plasmid
    #58224
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    hygromycin B phosphotransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58224-plasmid-map-sequence-id-89706

    Enlarge

  17. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

    Enlarge

  18. mEmerald-GFAP-C-18

    Plasmid
    #54106
    Purpose
    Localization: Intermediate Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    GFAP (GFAP Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. mWasabi-Vimentin-7

    Plasmid
    #56511
    Purpose
    Localization: Intermediate Filaments, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. PTK2BA

    Plasmid
    #42401
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTK2BA (PTK2B Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. mApple-Vimentin-7

    Plasmid
    #54960
    Purpose
    Localization: Intermediate Filaments, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. mRuby-Vimentin-7

    Plasmid
    #55884
    Purpose
    Localization: Intermediate Filaments, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. Dronpa-Vimentin-C-18

    Plasmid
    #57306
    Purpose
    Localization: Intermediate Filaments, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pFGL822

    Plasmid
    #58225
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    58225-plasmid-map-sequence-id-115839

    Enlarge

  32. sfGFP-Vimentin-7

    Plasmid
    #56489
    Purpose
    Localization: Intermediate Filaments, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. tdTomato-Vimentin-7

    Plasmid
    #58143
    Purpose
    Localization: Intermediate Filaments, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. PHPT1A

    Plasmid
    #42360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHPT1A (PHPT1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. PBC002-A04

    Plasmid
    #58761
    Purpose
    bacterial expression of PDB 4PWY
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PWY (PEX6 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. Dronpa-Vimentin-7

    Plasmid
    #57305
    Purpose
    Localization: Intermediate Filaments, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

    Enlarge

  41. pFGL822-8

    Plasmid
    #58226
    Purpose
    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.
    Depositor
    Naweed Naqvi
    Article
    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,
    Insert
    phosphinothricin acetyl transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    May 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    58226-plasmid-map-sequence-id-89710

    Enlarge

  43. pRL1075

    Plasmid
    #70688
    Purpose
    Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain
    Depositor
    Peter Wolk
    Article
    Black et al Mol Microbiol. 1993 Jul;9(1):77-84.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70688-plasmid-map-sequence-id-126455

    Enlarge

  44. 4QQN

    Plasmid
    #61951
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HRMT1L3:APC035-H01:C21507 (PRMT3 Human)
    Use
    Tags
    6xHis-Thrombin and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. mAmetrine-Vimentin-7

    Plasmid
    #56551
    Purpose
    Localization: Intermediate Filaments, Excitation: 406, Emission: 526
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mAmetrine
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56551-plasmid-map-sequence-id-216369

    Enlarge

  46. 4JDS

    Plasmid
    #60081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD7:DCC011-H01:C212434 (SETD7 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. mCerulean-Vimentin-7

    Plasmid
    #55395
    Purpose
    Localization: Intermediate Filaments, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  48. 4QQ4

    Plasmid
    #61945
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MORC3:JMC01B-H03:C203041 (MORC3 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. PIK3R2A

    Plasmid
    #42476
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIK3R2A (PIK3R2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. Dronpa-Vimentin-N-18

    Plasmid
    #57307
    Purpose
    Localization: Intermediate Filaments, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 11201 - 11250 of 13911 results