We narrowed to 22,939 results for: REP

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Human b2 nicotinic subunit crystallization construct

Plasmid

#85842

Purpose

pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct

Depositor

Ryan Hibbs

Article

Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

Insert

Human b2 nAChR subunit crystallization construct (CHRNB2 Human)

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

C-terminal StrepII tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-mOrange2 eFRET

Plasmid

#59173

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mOrange2 eFRET

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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p(bhsp68-Cas9)

Plasmid

#65959

Purpose

Tribolium basal heat shock promoter driving Cas9

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Insert

Cas9

Use

CRISPR

Tags

nuclear localization sequence

Expression

Insect

Promoter

bhsp

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET28CLY3

Plasmid

#21762

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pPos-Ma-CmR-TAG111

Plasmid

#197571

Purpose

Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

Chloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)

Expression

Bacterial

Mutation

111TAG and 8TAG/112TAG

Promoter

AraC, T7, cat promoter (constitutive), and lpp (c…

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS1096 Dual-sgRNA.Design 1

Plasmid

#159538

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 nuclease with two guide RNA cassettes with promoters

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA1-mRuby3

Plasmid

#184888

Purpose

Stable expression of ANXA1-mRuby3 (Annexin A1-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA1 (ANXA1 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-ER Long P2A

Plasmid

#164113

Purpose

Detect the long Peroxisomes-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP Po-ER Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSA110

Plasmid

#59784

Purpose

drives mCherry in C. elegans intestinal cells

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
mCherry (worm)
unc-54 3' UTR

Expression

Bacterial

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pTH-Cen18-mVenus

Plasmid

#49641

Purpose

TALEColor-mVenus-Chromosome 18-specific Centromere

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TALEColor-Cen18

Use

TALEN

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRU1098

Plasmid

#14463

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

unstable gfp-mut3.1 LAA

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-EEA-5guides-PGK-Puro

Plasmid

#102898

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5guides-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Dmrt1

Plasmid

#41083

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Dmrt1 (Dmrt1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_roGFP2_Orp1

Plasmid

#155043

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS- HIV2-ROD vpx

Plasmid

#115816

Purpose

Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV2ROD Vpx (M15390)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-hPPT1

Plasmid

#85815

Purpose

Expression of hPPT in mammalian cells

Depositor

Sandra Hofmann

Article

Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

Insert

human PPT1 (PPT1 Human)

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY546

Plasmid

#90466

Purpose

Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-mcherry

Tags

mcherry

Expression

Worm

Promoter

Pmec-4

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase

Plasmid

#163404

Purpose

Expresses BMPR-2 lacking the kinase domain in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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