We narrowed to 11,278 results for: VARS

1. pAd GFP HDAC5 S259A/S498A

   Plasmid

   #32218

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Adenoviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S259A/S498A

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pTA231-p.Syn-Dendra2Hfx

   Plasmid

   #164660

   Purpose

   expression of H. volcanii codon-optimized Dendra2

   Depositor

   Anita Marchfelder

   Article

   Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

   Insert

   Dendra2Hfx
   

   Use

   Archaeal expression

   Mutation

   codon-optimization for Haloferax volcanii

   Promoter

   p.Syn

   Available Since

   Feb. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. sg1+sg2+sg3

   Plasmid

   #113969

   Purpose

   Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1+sg2+sg3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

   Plasmid

   #168135

   Purpose

   Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoV2-N-M234I

   Plasmid

   #177955

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (M234I) (N )
   

   Expression

   Mammalian

   Mutation

   M234I

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-rtTA-zeo

   Plasmid

   #183233

   Purpose

   Lentiviral vector expressing the rtTa gene

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   rtTA-T2A-Zeo
   

   Use

   Lentiviral

   Promoter

   UBC

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_NOXO1-1/2

   Plasmid

   #91499

   Purpose

   Protein expression and purification of human SH3 domain construct NOXO1-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NOXO1-1/2 (NOXO1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_SHANK3-1/1

   Plasmid

   #91421

   Purpose

   Protein expression and purification of human SH3 domain construct SHANK3-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SHANK3-1/1 (SHANK3 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TTR C10S S85C K80D

   Plasmid

   #184743

   Purpose

   bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S, S85C, K80D

   Promoter

   T7

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNHT7::RPL15::hGluc

    Plasmid

    #186768

    Purpose

    An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_TNK1-1/1-SV1/3

    Plasmid

    #91548

    Purpose

    Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK1-1/1-SV1/3 (TNK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_SH3GL3-1/1

    Plasmid

    #91423

    Purpose

    Protein expression and purification of human SH3 domain construct SH3GL3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3GL3-1/1 (SH3GL3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR/U6 HDAC4 shRNA

    Plasmid

    #32220

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    shRNA against murine HDAC4 (Hdac4 Mouse)
    

    Use

    RNAi; Gateway u6 entry vector

    Promoter

    human U6

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

14. mWasabi-Mito-7

    Plasmid

    #56508

    Purpose

    Localization: Mitochondria, Excitation: 493, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Tags

    mWasabi

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pPKm-196

    Plasmid

    #90511

    Purpose

    pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF6-DBD
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR/U6 HDAC5 shRNA

    Plasmid

    #32222

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    shRNA against mouse HDAC5 (Hdac5 Mouse)
    

    Use

    RNAi; Gateway u6 entry vector

    Promoter

    human U6

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pFA0055

    Plasmid

    #131774

    Purpose

    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAd FLAG HDAC5 S259A/S498A

    Plasmid

    #32208

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S259A/S498A

    Available Since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159870

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p1.2-Zeo-eGFP

    Plasmid

    #162743

    Purpose

    Fluorescent reporter for CHO cells expression studies

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Insert

    enhanced green fluorescent protein
    

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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