164,577 results

1. pC0043-PspCas13b-gRNA non target

   Plasmid

   #223698

   Purpose

   gRNA control for dPspCas13b-FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for luciferase control
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MMW948: CAG-DmrC-NLS-3xFLAG-VPR

   Plasmid

   #104565

   Purpose

   Mammalian expression vector for DmrC fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   DmrC-VPR
   

   Use

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-rtTA

   Plasmid

   #126034

   Purpose

   PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

   Depositor

   Mauro Calabrese

   Article

   Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

   Insert

   rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbiC

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHJ12: CadC-VHH-Caffeine (No linker)

   Plasmid

   #108244

   Purpose

   Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO1

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BmTyr-Flag

   Plasmid

   #220779

   Purpose

   non-targeted BmTyr throughout the cytoplasm

   Depositor

   Itaru Hamachi

   Article

   Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

   Insert

   Bacillus megaterium Tyrosinase
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTHSSe_43

   Plasmid

   #109241

   Purpose

   pSC101 var1 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTHSSe_44

   Plasmid

   #109242

   Purpose

   pSC101 var2 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Potential silent point mutation in GFP sequence

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.2_mCherry_mito Grx1-roGFP2

    Plasmid

    #155044

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    mitochondrial targeting sequence and roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV-EF1amCherry-2A-OsTIR1-W

    Plasmid

    #159296

    Purpose

    Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    OsTIR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAK_DR30_CasRx_Puro

    Plasmid

    #134845

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A Puromycin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTHSSe_46

    Plasmid

    #109244

    Purpose

    pSC101 var4 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LeGO-iG

    Plasmid

    #27358

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, IRES-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5_EGFP-BRD4-NUT

    Plasmid

    #238254

    Purpose

    For overexpression of EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-BRD4-NUT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJFRC150-20XUAS-IVS-Flp1::PEST

    Plasmid

    #32132

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Flippase1::PEST
    

    Use

    Tags

    PEST

    Expression

    Insect

    Mutation

    Aspartic Acid at Amino Acid 5

    Promoter

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-neo beta catenin delta45

    Plasmid

    #16520

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    beta catenin (CTNNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta 45 (HCT116 mutation)

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pGFPGUSPlus

    Plasmid

    #64401

    Purpose

    binary plant vector expressing GFP and GUS, both driven by a 35S promoter

    Depositor

    Claudia Vickers

    Article

    Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

    Inserts

    GFP
    GUSPlus
    

    Use

    Binary vector for plant transformation

    Tags

    His

    Expression

    Plant

    Mutation

    S65T

    Promoter

    35S

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

    Plasmid

    #166055

    Purpose

    Expresses the full length TurboID on the surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. DP6

    Plasmid

    #140446

    Purpose

    Untargetted bacterial drift

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR ugi CDA1 gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge