We narrowed to 12,157 results for: pcDNA
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Plasmid#163099PurposeExpression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mEos3.2_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD438
Plasmid#168464PurposeFor the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
S113
Plasmid#174551PurposeConstitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)DepositorAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L plasmid with onboard mCherry cassette
Plasmid#169735PurposeEqualizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyExpressionMammalianPromoterCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
1010-AIDx-CP
Plasmid#135340PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorInsertCP 1010 nSpyCas9 -hAIDx
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
1058-AIDx-CP
Plasmid#135342PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.DepositorInsertCP 1058 nSpyCas9-hAIDx
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-208
Plasmid#135345PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9DepositorInsertnSpyCas9-hAIDx-ID-208
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-468
Plasmid#135346PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9DepositorInsertnSpyCas9-hAIDx-ID-468
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXD11wt-IDR
Plasmid#145242PurposeExpresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDRDepositorInsertHOXD11wt-IDR (HOXD11 Human)
ExpressionMammalianAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-MNX1-Adel-IDR
Plasmid#145247PurposeExpresses fusion of GAL4 DNA-binding domain and MNX1-Adel IDRDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-TBX1-Adel-IDR
Plasmid#145253PurposeExpresses fusion of GAL4 DNA-binding domain and TBX1-Adel IDRDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-delCry2
Plasmid#153545Purposethis is the non-clustering version of LIC-Z tagged with mcherryDepositorInserthuman TCR ζ-Chain (CD247 Human)
TagsmCherryExpressionMammalianMutationwith Cry2 sequence deleted from LIC-ZPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MEN2A-MAC
Plasmid#139634PurposeMAC-tagged gene expressionDepositorAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
GRB2-SHN
Plasmid#139625PurposeStrepIII-HA-tagged gene expressionDepositorAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3/gamma2SIL
Plasmid#121433PurposeGABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1/gamma2SIL
Plasmid#121432PurposeGABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR152
Plasmid#106052PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR152; Opto-GPR152; E6)DepositorInsertOpto-GPR152 (GPR152 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR176
Plasmid#106060PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)DepositorInsertOpto-GPR176 (GPR176 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR183
Plasmid#106062PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR183; Opto-GPR183; F4)DepositorInsertOpto-GPR183 (GPR183 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR132
Plasmid#106042PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR132; Opto-GPR132; D8)DepositorInsertOpto-GPR132 (GPR132 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR141
Plasmid#106045PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)DepositorInsertOpto-GPR141 (GPR141 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR35
Plasmid#106018PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR35; Opto-GPR35; B8)DepositorInsertOpto-GPR35 (GPR35 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR50
Plasmid#106024PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR50; Opto-GPR50; C2)DepositorInsertOpto-GPR50 (GPR50 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR62
Plasmid#106028PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR62; Opto-GPR62; C6)DepositorInsertOpto-GPR62 (GPR62 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR15
Plasmid#106004PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR15; Opto-GPR15; A6)DepositorInsertOpto-GPR15 (GPR15 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR33
Plasmid#106016PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR33; Opto-GPR33; B6)DepositorInsertOpto-GPR33 (GPR33 Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PME-1_R369D mutant
Plasmid#119294PurposeMammalian overexpression of StrepII-tagged full length PPME1 with mutation in the PP2Ac binding motifDepositorAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-KSR R615H
Plasmid#118332PurposeExpression of mouse KSRDepositorAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 del3
Plasmid#96913PurposeMurine Atoh1 with amino acids 214-305 removedDepositorInsertAtoh1 (Atoh1 Mouse)
Tags2xFLAGExpressionMammalianMutationDeletion of amino acids 214-305Available SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Atoh1 del4
Plasmid#96914PurposeMurine Atoh1 with amino acids 306-347 removedDepositorInsertAtoh1 (Atoh1 Mouse)
Tags2xFLAGExpressionMammalianMutationDeletion of amino acids 306-347Available SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP-DCRD
Plasmid#87453Purposerapamycin-induced membrane-targeting of DCRDDepositorInsertDCRD (distal C-terminal regulatory domain) (CACNA1F Human)
Tags6xHis-T7, FKBP, and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP-PCRD
Plasmid#87452Purposerapamycin-induced membrane-targeting of PCRDDepositorInsertPCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
TagsFKBP and YFPExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RBPMS2
Plasmid#65747Purposeexpresses GFP-tagged RBPMS2 in mammalian cellsDepositorInsertRBPMS2 (RBPMS2 Human)
TagsGFPExpressionMammalianMutationS13N mutation in RBPMS2PromoterCMV, 2x TetAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_d23
Plasmid#59892Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutatedDepositorInsertmCherry with intron containing the mouse mir-124–3
TagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd and fourth seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_d3
Plasmid#59891Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.DepositorInsertmCherry with intron containing the mouse mir-124–3
TagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--3rd seed site removedPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-II+III-NES-mut
Plasmid#42043DepositorInsertTNRC6A (TNRC6A Human)
TagsEGFP and MycExpressionMammalianMutationdeleted aa716-772 and aa804-924, F951A, F955A, I9…PromoterCMVAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I+III-NES-mut
Plasmid#42042DepositorInsertTNRC6A (TNRC6A Human)
TagsEGFP and MycExpressionMammalianMutationdeleted aa457-532 and aa804-924, F951A, F955A, I9…PromoterCMVAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-I+II-NES-mut
Plasmid#42039DepositorInsertTNRC6A (TNRC6A Human)
TagsEGFP and MycExpressionMammalianMutationdeleted aa457-532 and aa716-772, F951A, F955A, I9…PromoterCMVAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-del.GW-III-NES-mut
Plasmid#42041DepositorInsertTNRC6A (TNRC6A Human)
TagsEGFP and MycExpressionMammalianMutationdeleted aa804-924, F951A, F955A, I958A, F960APromoterCMVAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
EphA2-YFP
Plasmid#108852PurposeEncodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linkerDepositorInsertEPHA2 (EPHA2 Human)
TagsLabeled with eYFP on the C-Terminus via a 15 amin…ExpressionMammalianAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tau (wt) -VC
Plasmid#87369Purposeexpresses hTau fused to the C-terminal part of VenusDepositorAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1-dmito-oROS-HT
Plasmid#216418PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.DepositorInsertdmito-oROS-HT
ExpressionMammalianPromoterCMVAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (wt)
Plasmid#87368Purposeexpresses hTau fused to the N-terminal part of VenusDepositorAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
TagsHA, HisExpressionMammalianPromoterCMVAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PC1575
Plasmid#232088PurposeMammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.DepositorInsertuAb with cloning Esp3I restriction site
TagsIRES-mCherryExpressionMammalianPromoterCMVAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only