We narrowed to 5,960 results for: actin

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pPBpuro-mNG-eDHFR(69K6)-p85iSH2

Plasmid

#172103

Purpose

Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-PRLH

Plasmid

#184618

Purpose

For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-PRLH

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-LTB

Plasmid

#184605

Purpose

For expression of a fluorescent sensor for leukotriene B4 in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-LTB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NKB

Plasmid

#184609

Purpose

For expression of a fluorescent sensor for neurokinin B in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NKB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NOC

Plasmid

#184611

Purpose

For expression of a fluorescent sensor for nociceptin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NOC

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJPF0190

Plasmid

#130268

Purpose

Piggybac vector for the expression of mRuby2-fused human PRKACA

Depositor

Hana El-Samad

Article

Fonseca et al bioRxiv 2020, 747030

Insert

PRKACA (PRKACA Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT077

Plasmid

#180511

Purpose

Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
PlmCasX

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT526

Plasmid

#180513

Purpose

Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv1
PlmCasX-2A-mNeonGreen

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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B3GALT5_pLENTI-CAG-IRES-GFP

Plasmid

#177006

Purpose

Mammalian lentiviral expression vector encoding B3GALT5

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

B3GALT5 (B3GALT5 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CBR1_pLENTI-CAG-IRES-GFP

Plasmid

#177008

Purpose

Mammalian lentiviral expression vector encoding CBR1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CBR1 (CBR1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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CHAF1B_pLENTI-CAG-IRES-GFP

Plasmid

#177009

Purpose

Mammalian lentiviral expression vector encoding CHAF1B

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CHAF1B (CHAF1B Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

Plasmid

#182049

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A/T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CAG-Haunt-EF1a-DsRed

Plasmid

#92162

Purpose

Haunt in trans overexpression plasmid

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt (Halr1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PE NG

Plasmid

#174554

Purpose

All-in-one prime editor piggyBac transposon, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

Promoter

CAG

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1179

Plasmid

#179296

Purpose

Expresses dCas9 fused to four DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-DmrA(x4)

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1160

Plasmid

#179298

Purpose

Expresses dCas9 fused to P65

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-p65

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2

Plasmid

#178872

Purpose

Mammalian expression of PB1-MontiRed-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2dark

Plasmid

#178874

Purpose

Mammalian expression of PB1-MontiRed-LOV2dark

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2dark

Expression

Mammalian

Mutation

LOV2: C450A, L514K, G528A, L531E, N538E

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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