We narrowed to 7,271 results for: Tet-on

1. pBEAST-BenR

   Plasmid

   #114597

   Purpose

   "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   BenR
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Promoter

   OR2-OR1-Pr

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGLTR-FP-GFP

   Plasmid

   #58242

   Purpose

   GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   attR1-Cm(R)-CcdB-attR2
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Expression

   Mammalian

   Promoter

   no

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN12A-P750

   Plasmid

   #49525

   Purpose

   Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750
   

   Promoter

   P750

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

   Plasmid

   #217708

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL3 (ADGRL3 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-841

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MBP-TEVpCS-ADGRG5 CTF-FLAG

   Plasmid

   #217703

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG5 (ADGRG5 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-226

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-MBP-TEVpCS-ADGRF4 CTF-FLAG

   Plasmid

   #217697

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRF4 (ADGRF4 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-384

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP118

   Plasmid

   #105495

   Purpose

   pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusions

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

   Insert

   HupA-SmBiT/HupA-LgBiT
   

   Use

   Atc-dependent expression in c. difficile

   Promoter

   Ptet

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-STITCH

   Plasmid

   #129535

   Purpose

   STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   STITCH
   

   Use

   CRISPR; Donor template

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-TRE-mTagBFP2-WPRE

   Plasmid

   #193332

   Purpose

   Expresses mTagBFP2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Mutation

   A XhoI site in the coding sequence is silenced

   Promoter

   TRE

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFU-cMVIIA-PE

    Plasmid

    #24147

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

    Insert

    loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid

    #133316

    Purpose

    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus pyogenes)
    

    Use

    CRISPR

    Promoter

    xylose

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Promoter

    J23101

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pyc128m

    Plasmid

    #158778

    Purpose

    soluble GCaMP expression

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5A0-T

    Plasmid

    #92016

    Purpose

    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Promoter

    J23102 Promoter (from Anderson promoter library) …

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b – E. coli RecE residues 564-868

    Plasmid

    #104534

    Purpose

    Expresses E. coli RecE residues 564-868

    Depositor

    Charles Bell

    Article

    Zhang et al Structure. 2009 May 13;17(5):690-702. do

    Insert

    RecE residues 564-866 (C-terminal nuclease domain)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIneo-HA-Ago2

    Plasmid

    #115362

    Purpose

    Expression vector to produce HA-tagged human Ago2 protein without N-peptide

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al RNA. 2004 Oct . 10(10):1518-25.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. GB1-His-SAP25-126-186(pL91)

    Plasmid

    #124387

    Purpose

    E.Coli produced SAP25 SID purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-SAP25-126-186 (SAP25 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)

    Plasmid

    #36879

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHL 651

    Plasmid

    #53016

    Purpose

    LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    LacIq
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and T1 terminator

    Expression

    Bacterial

    Promoter

    pCon

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only