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pCAG-CaBLAM_294W

Plasmid

#244130

Purpose

Lower affinity variant of CaBLAM bioluminescent calcium indicator

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM_294W

Expression

Mammalian

Mutation

Calmodulin N98W

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-CaBLAM_332W

Plasmid

#244131

Purpose

Higher affinity variant of CaBLAM bioluminescent calcium indicator

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM_332W

Expression

Mammalian

Mutation

Calmodulin Q135W

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet_6xHis-TEV-mCherry-ATG13 IDR(230-517aa)

Plasmid

#227711

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

mCherry-ATG13 IDR(230-517aa) (ATG13 Human)

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-EGFP-ATG13_IDR (191-205aa)

Plasmid

#223791

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-205 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-EGFP-ATG13_IDR (191-230aa)

Plasmid

#223792

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-230 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-EGFP-ATG13_IDR (206-230aa)

Plasmid

#223793

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 206-230 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

Plasmid

#223778

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR_delta191-230 (231-517aa)

Plasmid

#223785

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 231-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR_delta191-204 (205-517aa)

Plasmid

#223786

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 205-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR_delta206-230 (191-205_231-517aa)

Plasmid

#223787

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

expresses aa 191-517, with 206-230 deleted

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

Plasmid

#223766

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

Plasmid

#223738

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A mLIR

Plasmid

#215506

Purpose

Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVss-U6-sgHTT51-7sk-Cas9

Plasmid

#190901

Purpose

AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA (Htt Mouse)

Use

AAV and CRISPR

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a-KRAB_crRNA Td2

Plasmid

#176261

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

Kruppel associated box domain and luciferase

Mutation

D917A and E1006A mutations to inactivate the endo…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-F241S

Plasmid

#140740

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENF241S (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

F241S

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-W274L

Plasmid

#140742

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation W274L and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENW274L (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

W274L

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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