We narrowed to 17,632 results for: URE
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Plasmid#75298PurposeExpresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 1-339
TagsGST-TagExpressionBacterialAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL0746 (pDonor_R-MmeI)
Plasmid#130646PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (R*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATA --> TGTTGGAAAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N
Plasmid#113952Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S and S212N su…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S D808N
Plasmid#169947PurposePurification of catalytically-dead DPP9S from insect cellsDepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
prk5-TMEM192-3xHA PM mutant
Plasmid#149506PurposeTransient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.DepositorInsertTMEM192 (TMEM192 Human)
UseTransient expressionTags3XHAExpressionMammalianMutationp.LL23-24AA, p.LL28-29AAPromoterCMVAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII
Plasmid#209292PurposeExpresses R2Bm-Cas9(H840A) fusion in E. coliDepositorInsertR2
TagsHis, MBP, SpCas9(H840A), StrepII, and bdSUMOExpressionBacterialPromoterT7Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS10
Plasmid#113956Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitution…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1l
Plasmid#214817PurposeBacterial expression of GST-tagged mouse GTSF1lDepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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HNRNPH2_3xRRM_pGEX
Plasmid#135115PurposeRecombinant expression of HNRNPH2 with dual-affinity tagDepositorAvailable SinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
A*01:03:01
Plasmid#203293PurposeOverexpress HLA alleleDepositorInsertA*01:03:01 (HLA-A Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-EIF3B-Reverse-Complement
Plasmid#136016PurposeThe Reverse Complement of EIF3B 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ArpC4_pLib
Plasmid#173681PurposeArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO BROX 1-411;
Plasmid#89602PurposeExpresses full length human Brox in bacteria; internal ID WISP11-297DepositorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF711-pET28a-Hs-delta25-MCAD-NHis wt
Plasmid#85112Purposeexpression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP4-1/1
Plasmid#91518PurposeProtein expression and purification of human SH3 domain construct ARHGAP4-1/1DepositorInsertARHGAP4-1/1 (ARHGAP4 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP9-1/1
Plasmid#91442PurposeProtein expression and purification of human SH3 domain construct ARHGAP9-1/1DepositorInsertARHGAP9-1/1 (ARHGAP9 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72P)
Plasmid#62083PurposeExpression of human p53 (1-73) (72P) in e. coliDepositorInsertp53 (TP53 Human)
TagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72PPromoterT7Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CEP55_EABR
Plasmid#178469PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A66V
Plasmid#166817PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)DepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP
Plasmid#136005PurposeDOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertG3BP1 (G3BP1 Human)
TagsEGFPExpressionMammalianMutationC-term of G3BP1 Deleted for Delta RBPAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR
Plasmid#75136PurposeRetroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
Tags6His-TEVExpressionBacterialMutationR49APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG23 RELA 3'UTR
Plasmid#14505DepositorAvailable SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD2b
Plasmid#109517PurposeMuscle specific zebrafish expression of mKate2 fused to EHD2bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-pEGFP-C1
Plasmid#166737PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BikDepositorAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-iRFP670 L1-R5
Plasmid#84390PurposeMultisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsiRFP670ExpressionMammalianPromoterCMV immediate earlyAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-Stim1-D76A
Plasmid#186622PurposeEncodes Stim1 mutant D76A fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
TagsYFPExpressionMammalianMutationD76APromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3
Plasmid#49822Purposefor transcribing SRA nucl 419-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR
Plasmid#75135PurposeRetroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EPS15_EH
Plasmid#178475PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FUT8-1/1
Plasmid#91270PurposeProtein expression and purification of human SH3 domain construct FUT8-1/1DepositorInsertFUT8-1/1 (FUT8 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
B*07:05:01
Plasmid#203268PurposeOverexpress HLA alleleDepositorInsertB*07:05:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn4a-NTD1-135-mNeon
Plasmid#186609PurposeEncodes Reticulon 4 N-terminal domain aa 1 to 135 fused to mNeonGreenDepositorInsertHuman Reticulon 4 isoform A amino acids 1 to 135 (RTN4 Human)
TagsmNeonGreenExpressionMammalianPromoterCMVAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CNOT4_RRM-ZNF_pGEX
Plasmid#135146PurposeRecombinant expression of CNOT4 with dual-affinity tagDepositorAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-foxc1a
Plasmid#132971Purposefull-length of foxc1a without stop codon in pmE Gateway vectorDepositorInsertfoxc1a (foxc1a Zebrafish)
UseGateway vectorAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
NET97 pmRFP-N2 (641)
Plasmid#61994Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
ArpC5_pLib
Plasmid#173682PurposeArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC1_pLib
Plasmid#173678PurposeArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
STK16_HUMAN_D0
Plasmid#79701PurposeThis plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
TagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 P1214R
Plasmid#166822PurposeGateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(767-1356)
Plasmid#49826Purposefor transcribing SRA nucl 767-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
ExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_S212N
Plasmid#113951Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitutionPromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EEA1
Plasmid#109508PurposeMuscle specific zebrafish expression of mKate2 fused to early endosome associated 1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG17 IQGAP1 3'UTR
Plasmid#14503DepositorAvailable SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2
Plasmid#85667PurposeExpression of human YOD1 I292Q V295Q mutant with N-terminal GFP tagDepositorAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(2Q)
Plasmid#21752DepositorInsertAtaxin-1 (ATXN1 Human)
TagsGSTExpressionMammalianMutationinsert contains 2Q (2 glutamines) and there isno …PromoterCMVAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only