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pFOSGE_COF2H09

Plasmid

#70924

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mnn1 (Mnn1 Fly)

Use

Gateway Cloning

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pME-Fv2E-flt4-ns

Plasmid

#67695

Purpose

middle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4

Mutation

no stop codon; D1302E (see depositor's comme…

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only
pENTR2B-dCas9-KRAB

Plasmid

#115547

Purpose

Entry cassette for dCas9-KRAB

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Insert

dCas9-KRAB

Tags

HA

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcLF1

Plasmid

#198581

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcLF1

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BOLF1

Plasmid

#198579

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BOLF1

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-SM

Plasmid

#195814

Purpose

Gateway Entry Clone of Epstein-Barr virus SM

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

SM

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3XFlag-WT-MEFV

Plasmid

#167018

Purpose

Gateway entry plasmid with 3xflag-pyrin (Wild-type)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway Cloning

Tags

3xFlag

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK001

Plasmid

#110573

Purpose

Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcs2-GFP-IRES-mCherry

Plasmid

#226098

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj14

Plasmid

#173144

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj14 (kcnj14 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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TurboID-mNeongreen-3xFLAG fusion pos3

Plasmid

#172864

Purpose

C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

Depositor

Mario de Bono

Article

Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

Insert

TurboID

Use

Unspecified

Tags

FLAG, mNeongreen, and myc

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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[Bla][IRES][GFP]

Plasmid

#97193

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSW177 - pCASP1

Plasmid

#115986

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Alexis Maizel

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCASP1

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ153

Plasmid

#69304

Purpose

Gateway entry vector with pco-dCas9-3X(SRDX)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-3X(SRDX) (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

SRDX (X3) repressor fusion; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP

Plasmid

#75175

Purpose

TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ131A2.0

Plasmid

#99884

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132A2.0

Plasmid

#99887

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ133A2.0

Plasmid

#99891

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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