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Plasmid#113342PurposeExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertdCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast EF1a
Plasmid#206223PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-tetO-EF1a-GFP
Plasmid#187645PurposeLentiviral vector expressing GFP from EF1a promoter downstream from tetO sitesDepositorInsertGFP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.10_M3_Ef1a-HA-gateway-SV40
Plasmid#128446Purposelentiviral destination vector with Ef1a promoter, N-terminal HA tag, and SV40 promoterDepositorInsertEf1a promoter, N-terminal HA tag, and SV40 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-EF1a-Puro
Plasmid#127170PurposePCR template for EF1a-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-Itprip-V5
Plasmid#120241PurposeExpresses V5-tagged Itprip in lentiviral vectorDepositorInsertItprip (ITPRIP Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1-P2A-Hygro_Barcode
Plasmid#170221PurposeBarcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97312PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.DepositorInsertActb HMEJ donor
UseLentiviral and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CCNB1-P2A-Hygro_Barcode
Plasmid#170218PurposeBarcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCCNB1 (CCNB1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Sec61B
Plasmid#120244PurposeExpresses V5-tagged Sec61B in lentiviral vectorDepositorInsertSec61B (Sec61b Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CDreV
Plasmid#140133Purposecan be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomerDepositorInsertCDreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.11_M3_Ef1a-gateway-HA-SV40
Plasmid#128447Purposelentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoterDepositorInsertEf1a promoter, C-terminal HA tag, and SV40 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-2xSV40_NLS-CeNLuc
Plasmid#135952PurposeExpresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseTags2xSV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-KLF4
Plasmid#35389DepositorInsertTALE-Klf4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159
Plasmid#170170PurposeLentiviral Expression of EF1a.SLC6A1.WTDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1alpha-HA-TFAP4
Plasmid#110158PurposeLentiviral vector for expression of human TFAP4 in mammalian cellsDepositorInsertTFAP4 (transcription factor AP-4) (TFAP4 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-HB
Plasmid#37452DepositorInsertHistone, 2A element, rabies B19 glycoprotein
UseAdeno-associated vectorTagsGFPExpressionMutationPromoterEF1aAvailable sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-QCP-3xBFP
Plasmid#158206PurposeQb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.DepositorInsertQCP under the Elfa promoter and three BFP tags
UseSynthetic BiologyTags3xBFPExpressionMammalianMutationPromoterElfaAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -