We narrowed to 7,566 results for: ef1a;

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->GssmCherry-PNE

Plasmid

#162157

Purpose

Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113343

Purpose

Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

p300core-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9GFP-W

Plasmid

#200100

Purpose

Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

CRISPR and Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtLaG2

Plasmid

#162224

Purpose

Lentiviral vector for constitutive expression of Myc GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1dn (og codon)

Plasmid

#174823

Purpose

Mammalian expression of human MLH1dn (original codon usage)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (original codon usage) (MLH1 Human)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEF1a-Usp9x_CD-mCherry

Plasmid

#170380

Purpose

Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

Depositor

Miguel Ramalho-Santos

Article

Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

Insert

Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)

Tags

mCherry-3xFlag

Expression

Mammalian

Mutation

Catalytic domain

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-ZsGreen1_Blast

Plasmid

#192660

Purpose

3rd generation lenti vector encoding ZsGreen1 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Insert

ZsGreen1

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' KDM5C

Plasmid

#236670

Purpose

5' AAVLINK plasmid for KDM5C expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

KDM5C (KDM5C Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-dsRed-W

Plasmid

#195863

Purpose

lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

Depositor

Darrell Kotton

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Promoter

EF1aL

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' IQGAP3

Plasmid

#236704

Purpose

5' AAVLINK plasmid for IQGAP3 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

IQGAP3 (IQGAP3 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-L4R1-hEF1a

Plasmid

#139523

Purpose

Provides constitutive promoter cassette between attL4-attR1 on Gateway entry vector

Depositor

Minoru Ko

Insert

hEF1a promoter

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-CCNE2

Plasmid

#162554

Purpose

Overexpression of CCNE2

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE2 (CCNE2 Human)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->sGFP_PGK-->puroR

Plasmid

#162156

Purpose

Lentiviral vector for constitutive expression of GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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