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pαH-NiV-F-WT-Foldon

Plasmid

#200391

Purpose

Mammalian cell expression of Nipah virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-LayV-F-WT-Foldon

Plasmid

#200392

Purpose

Mammalian cell expression of Langya virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

LayV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-483)

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-3HB-mutant

Plasmid

#237852

Purpose

DriD 3HB surface mutant used to interrogate the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 3HB mutant

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-Nluc

Plasmid

#192659

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, TRE3G

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-Nluc

Plasmid

#192657

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K

Plasmid

#112652

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K

Expression

Yeast

Mutation

R59K (GAP-dead)

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K

Plasmid

#112647

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K

Expression

Yeast

Mutation

R54K

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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KA2963_eSpCas9-PGKHygdtkCh

Plasmid

#124205

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

Plasmid

#124169

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1123_pPB-TREtight-cHA-IRESH2BmKO2

Plasmid

#124172

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a Q146F

Plasmid

#75259

Purpose

Q146F mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a

Tags

His

Expression

Yeast

Mutation

Q146F

Promoter

Met25

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a T158K

Plasmid

#75262

Purpose

T158K mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a T158K

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a K153Q

Plasmid

#75261

Purpose

K153Q mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a K153Q

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a N152T

Plasmid

#75260

Purpose

N152T mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a N152T

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-GCN4cc-Erp2CT

Plasmid

#73923

Purpose

GST fusion- coiled coil- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1- Mst7-Erp2CT

Plasmid

#73922

Purpose

GST fusion- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-GCN4cc-Erp1CT

Plasmid

#73921

Purpose

GST fusion- coiled coil- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1- Mst7-Erp1CT

Plasmid

#73920

Purpose

GST fusion- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 δCLD

Plasmid

#73876

Purpose

δCLD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD

Plasmid

#73875

Purpose

Ret2LD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD2α

Plasmid

#73873

Purpose

Ret2LD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2 I155A

Plasmid

#73888

Purpose

I155A mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 I155A

Expression

Yeast

Mutation

Ile 155 Ala

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 Ret2 I149A

Plasmid

#73887

Purpose

I149A mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 I149A

Expression

Yeast

Mutation

Ile 149 Ala

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-15

Plasmid

#73884

Purpose

Chimaera 15

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-15

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-14

Plasmid

#73883

Purpose

Chimaera 14

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-14

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-13

Plasmid

#73882

Purpose

Chimaera 13

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-13

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-12

Plasmid

#73881

Purpose

Chimaera 12

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-12

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-10

Plasmid

#73880

Purpose

Chimaera 10

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-10

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-9

Plasmid

#73879

Purpose

Chimaera 9

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-9

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-7

Plasmid

#73869

Purpose

Chimaera 7 (COPD-Ret2)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Chimaera 7

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 CHI-1

Plasmid

#73863

Purpose

Chimaera 1 (Ret2-COPD)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Chimaera 1

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 Ret2

Plasmid

#73872

Purpose

Ret2 (full length)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2

Expression

Yeast

Promoter

Met25

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
miR766 in pcDNA3.1

Plasmid

#78124

Purpose

Mammalian expression of miR766

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR766 (MIR766 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVD437

Plasmid

#63758

Purpose

7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)

Depositor

James Kaper

Article

Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

Insert

eae

Expression

Bacterial

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-EF1alpha-dCas9-10xGCN4_Hygro

Plasmid

#192651

Purpose

3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-10xGCN4

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML1-HA

Plasmid

#18825

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mt POLRMT

Plasmid

#205059

Purpose

POLRMT protein expression

Depositor

Smita Patel

Article

Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

Insert

POLRMT (POLRMT Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

Plasmid

#240411

Purpose

This plasmid facilitates the assessment of MMR proficiency in human living cells

Depositor

Isao Kuraoka

Article

Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

Insert

NLS-EGFP fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPML1-YFP

Plasmid

#18826

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

YFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
PAmCherry1-N1-Tubbyc

Plasmid

#202677

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505): PAmCherry1 (Tub Mouse)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-RosaR4 KKR mutations

Plasmid

#37199

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to RosaR4 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs R4, and SV40 NLS

Expression

Mammalian

Mutation

I538K, E490K, and H537R

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-RosaL6 ELD mutations

Plasmid

#37198

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to Rosa L6 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs L6, and SV40 NLS

Expression

Mammalian

Mutation

Q486E, I499L and N496D

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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