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pcDNA-FLAG-Mo-Trip6

Plasmid

#27254

Depositor

Thomas Gilmore

Article

Wang et al Gene. 1999 Jul 8. 234(2):403-9.

Insert

Trip6 (Trip6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
pGWT35S-MpPHOT-Citrine

Plasmid

#220106

Purpose

Transient expression of MpPHOT-Citrine in plant cells

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-Citrine

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOVSD

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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TRIP12-V2_A755-A841

Plasmid

#194759

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

Insert

TRIP12 (TRIP12 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTRIPZ-p21-HA(RxL)

Plasmid

#215111

Purpose

Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

Promoter

CMV (Tet inducible)

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST53-mTrific

Plasmid

#51031

Purpose

mammalian expression of mouse Trific fused to GFP

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

Trific (Trim46 Mouse)

Use

Tags

Cycle3 GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin3

Plasmid

#72592

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN3 (SESN3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

ProRUBY

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCasper4-g-osk-TRICK

Plasmid

#71244

Purpose

oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

oskar (osk Fly)

Use

P element-mediated germline transformation

Tags

12xPP7 and 6xMS2

Expression

Insect

Mutation

Promoter

osk

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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hSyn-Aion-ts-citrine-ER

Plasmid

#192575

Purpose

AAV-vector for optogenetic long-term silencing of neurons

Depositor

Simon Wiegert

Article

Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

Insert

Aion

Use

AAV

Tags

ts-Citrine-ER

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

human synapsin

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_TRIB1:1-372

Plasmid

#210969

Purpose

Mammalian expression of full-length human TRIB1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TRIB1:M1-C372 (TRIB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EAAARx8-CaaX

Plasmid

#155224

Purpose

MPAct with increased search radius

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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GST-TRIM24+TEV

Plasmid

#28147

Depositor

Michelle Barton

Insert

TRIM24 (TRIM24 Human)

Use

Tags

GST and TEV cleavage site

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pTripz NEO DEST

Plasmid

#209072

Purpose

Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

Depositor

Matthew Oser

Article

Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

Type

Empty backbone

Use

Lentiviral; Gateway destination vector to be used…

Tags

3X FLAG

Expression

Mutation

Promoter

CMV

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_TRIB1:1-372

Plasmid

#210968

Purpose

Mammalian expression of full-length human TRIB1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

TRIB1:M1-C372 (TRIB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mem-mCherry-pTRIP

Plasmid

#163521

Purpose

Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

Depositor

Thomas Weber

Insert

membrane targeted mCherry

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXa3

Plasmid

#73614

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXa3

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Chicken B-Actin

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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