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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-Synaptojanin-1-145

Plasmid

#223563

Purpose

Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

SYNJ1 (SYNJ1 Human)

Use

Tags

His and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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alpha5-EGFP

Plasmid

#118956

Purpose

GABAA receptor expression (alpha5 subunit). EGFP tag at the C-terminus.

Depositor

Angel de Blas

Insert

GABRA5 (Gabra5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-human OCRL

Plasmid

#22207

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Oculocerebrorenal syndrome of Lowe (OCRL Human)

Use

Retroviral

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2 GSK3-GFP

Plasmid

#29680

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
Myc-E-Syt2

Plasmid

#66834

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
p3xFLAG CMV14 / BRAF-G469V

Plasmid

#131718

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

G469V

Promoter

CMV

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1isoform2-GFP

Plasmid

#224589

Purpose

For expression of FAM177A1-GFP in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-170

Plasmid

#22292

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Use

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

K334R compared to Genbank ID NM_003895

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt3

Plasmid

#66835

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt3 (ESYT3 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-INPP5E

Plasmid

#223564

Purpose

Expresses the full-length mouse INPP5E (Pharbin)

Depositor

Pietro De Camilli

Article

Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

INPP5E (Inpp5e Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2

Plasmid

#16066

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

aa2-500, inserted alanin at aa434

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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VPS13B^GFP

Plasmid

#224588

Purpose

codon optimized GFP tagged VPS13B for expression in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

VPS13B^GFP (VPS13B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 GSK3-RFP

Plasmid

#29679

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
GFP-Dynamin 2 K44A

Plasmid

#22301

Depositor

Pietro De Camilli

Article

Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Insert

Dynamin 2 K44A (Dnm2 Rat)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
HA-mDyn1 pcDNA3

Plasmid

#36263

Depositor

Pietro De Camilli

Article

Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

Insert

Dynamin 1 (Dnm1 Mouse)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Synaptojanin 1-145

Plasmid

#22291

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Use

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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BLBP-mCherry

Plasmid

#63721

Purpose

Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1700 bp upstream of BLBP gene cloned in CAG-mCher…

Promoter

1700bp upstream of BLBP gene

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

Plasmid

#158742

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/354/16 H3N2

Use

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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