We narrowed to 1,376 results for: piggyBac

1. pPB-3xnls-Tq-Ca-FLITS

   Plasmid

   #145030

   Purpose

   piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-Tq-Ca-FLITS
   

   Tags

   3xnls-Tq-Ca-FLITS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-Neo-TetO-Cdk12_Nterm_FlagHa

   Plasmid

   #127179

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mammalian

   Promoter

   TetO Promoter

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

   Plasmid

   #216763

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40

   Plasmid

   #186969

   Purpose

   PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   Dnmt3a2 (Dnmt3a Mouse, Synthetic)
   

   Use

   Piggybac

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Isoform 2 (residues 220–908)

   Promoter

   CAG

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-gRNA-TAZ

   Plasmid

   #86688

   Purpose

   Expresses a gRNA targeted to human TAZ

   Depositor

   William Pu

   Article

   Wang et al Nat Protoc. 2017 Jan;12(1):88-103. doi:

   Insert

   U6-gRNA-TAZ (TAFAZZIN Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-iRFP-STOP-ReNL

   Plasmid

   #113965

   Purpose

   Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

   Depositor

   Jordan Green

   Article

   Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

   Inserts

   iRFP670
   ReNL
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   CMV - SV40-PolyA - SV40-PolyA and EF1A

   Available Since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-U6-CNCB_sgNANOG

   Plasmid

   #242176

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven NANOG sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   NANOG (NANOG Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-U6-CNCB_sgSOX2

   Plasmid

   #242175

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven SOX2 sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-U6-CNCB_sgPOU5F1

   Plasmid

   #242174

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven POU5F1sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

    Plasmid

    #236173

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
    H2B (H2BC11 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and four ZNF35/ZNF35 sites

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

    Plasmid

    #236172

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    H2B (H2BC11 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and four ZNF35/ZNF250 sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid

    #236174

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and four ZNF35/IKZF1 sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

    Plasmid

    #233388

    Purpose

    PiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    IL2Rb(ecto+TM)-ADAR2dd
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

    Plasmid

    #229995

    Purpose

    PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

    Depositor

    Christian Mayer

    Article

    Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NotI site for NEBuilder HiFi DNA Assembly with ss…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB9P2

    Plasmid

    #210030

    Purpose

    Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB9P3

    Plasmid

    #210034

    Purpose

    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC2.4

    Plasmid

    #226557

    Purpose

    NATURA gene encoding plasmid

    Depositor

    Dave Wee

    Article

    Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

    Insert

    NATURA reporter gene
    

    Use

    Luciferase; Piggybac

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPN432

    Plasmid

    #137870

    Purpose

    Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN431

    Plasmid

    #137869

    Purpose

    Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN435

    Plasmid

    #137867

    Purpose

    Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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