162,981 results

1. pCDH-EF1a-hSNCA

   Plasmid

   #170445

   Purpose

   Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   human SNCA (SNCA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLXSN16E6E7

   Plasmid

   #52394

   Purpose

   The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

   Depositor

   Denise Galloway

   Article

   Halbert et al J Virol. 1991 Jan;65(1):473-8.

   Insert

   HPV16 E6E7
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. MI-Luciferase-IRES-mCherry

   Plasmid

   #75020

   Purpose

   Encodes the expression of Luciferase and mCherry

   Depositor

   Xiaoping Sun

   Article

   Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

   Inserts

   firefly Luciferase
   mCherry
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHM6-alphasynuclein-WT

   Plasmid

   #40824

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti GFP-AMOT p130

   Plasmid

   #32828

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

   Insert

   AMOT p130 (AMOT Human)
   

   Use

   Lentiviral

   Tags

   GFP hAMOT

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM2 hTSP-1

   Plasmid

   #12993

   Depositor

   Jack Lawler

   Insert

   TSP-1 (THBS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSens-4OmeNB

   Plasmid

   #216231

   Purpose

   Expresses GFP in response to 4'-O-Methylnorbelladine

   Depositor

   Andrew Ellington, Simon d'Oelsnitz

   Article

   d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

   Inserts

   RamR-4NB2
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   K64T, L67M, C135D, S138G

   Promoter

   J23103 and Pramr

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSyn.iGluSnFR3.v857.SGZ

   Plasmid

   #178330

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. enIscB

    Plasmid

    #205410

    Purpose

    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E85R+H369R+S387R+S457R

    Promoter

    CAG, hU6, CMV

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 Flag MKK7B2Jnk2a2

    Plasmid

    #19727

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGPD2

    Plasmid

    #43972

    Depositor

    Hal Alper

    Article

    Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPD

    Available since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p4947 pcDNA4c hBrd4 full-length

    Plasmid

    #14441

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (BRD4 Human)
    

    Use

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-T7-6h-GST-α-Synuclein

    Plasmid

    #225224

    Purpose

    Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    GST
    α-Synuclein
    

    Use

    Tags

    6xHis Tag and TEV Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-tetO-NGN2

    Plasmid

    #197090

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-EOS-GFP-IP

    Plasmid

    #60439

    Purpose

    PiggyBac for EOS-GFP

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    EOS
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3 Flag MKK7B2Jnk1a1(APF)

    Plasmid

    #19730

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif Thr(1959)-Pro-…

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-sadCas9-VP64

    Plasmid

    #115790

    Purpose

    An AAV vector expressing S. aureus dCas9 fused to VP64.

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    Sa-dCas9
    

    Use

    AAV

    Tags

    NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

    Plasmid

    #214926

    Purpose

    Expresses presynaptic non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).cpsfGFP.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. p663-UBC-miniTurbo-V5-PKN3_IDG-K

    Plasmid

    #170601

    Purpose

    Gateway destination clone of PKN3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PKN3 (PKN3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PL-SIN-PGK-EiP

    Plasmid

    #21312

    Purpose

    Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ITGA6-bio-His

    Plasmid

    #53352

    Purpose

    Expresses full-length Integrin alpha-6 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ITGA6 (ITGA6 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-28a(+)-FGF2-K128N

    Plasmid

    #120284

    Purpose

    Produce human recombinant FGF2 K128N in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3.1 hSCN5A

    Plasmid

    #145374

    Purpose

    expresses human Nav1.5 (WT) in mammalian cells

    Depositor

    Robert Kass, Hugues Abriel

    Article

    Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge