165,343 results

1. pmGFP-ADAR2

   Plasmid

   #117929

   Purpose

   ADAR2 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR2 (ADARB1 Human)
   

   Tags

   myc-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT_TO_FlagHA_G3BP1

   Plasmid

   #127106

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP1 (G3BP1 Human)
   

   Expression

   Mammalian

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. DRD2-Tango

   Plasmid

   #66269

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   DRD2 (DRD2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LARS_pNIC-Bio3

   Plasmid

   #153052

   Purpose

   Expression construct for producing Avi tagged Leucyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   LARS (LARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Not full length protein

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXB1-nbOcIgG-DddA11_NT

   Plasmid

   #232441

   Purpose

   Anti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

   Depositor

   Ivan Raimondi

   Article

   Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

   Insert

   nbOcIgG-DddA11_N
   

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ150

   Plasmid

   #69301

   Purpose

   Gateway entry vector with pcoCas9 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pcoCas9 (plant codon-optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Mutation

   NLS fusion

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200630

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SF3B3-1

   Plasmid

   #156004

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SF3B3 (SF3B3 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Xl-VSP1

    Plasmid

    #51880

    Purpose

    expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR58.6

    Plasmid

    #63176

    Purpose

    Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

    Plasmid

    #172982

    Purpose

    control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    control sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_CBR1_WT_V5

    Plasmid

    #82945

    Purpose

    Gateway Donor vector containing CBR1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CBR1 (CBR1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-CD80

    Plasmid

    #223603

    Purpose

    Lentiviral expression of human CD80 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Promoter

    cx55.5

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUX19

    Plasmid

    #36328

    Depositor

    Gary Roberts

    Type

    Empty backbone

    Use

    Suicide vector for r. rubrum

    Expression

    Bacterial

    Promoter

    reduce strength lacZ

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

20. 1572 pSG5L Flag HA GGA2

    Plasmid

    #11184

    Depositor

    William Sellers

    Insert

    GGA2 (GGA2 Human)
    

    Tags

    HA and flag

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pSGNluc

    Plasmid

    #186705

    Purpose

    A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

    Depositor

    Maria Leptin, Baubak Bajoghli

    Article

    Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

    Inserts

    GFP
    luciferase
    

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV_2pabPAC

    Plasmid

    #158969

    Purpose

    2P-activatable bPAC for AAV

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPAC
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. TARS_pNIC-Bio3

    Plasmid

    #153057

    Purpose

    Expression construct for producing Avi tagged Threonyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    TARS (TARS1 Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-LSS-SGFP2

    Plasmid

    #112943

    Purpose

    Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Insert

    LSS-GFP2 mCherry fusion
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti-pHluorin_M153R-CD63

    Plasmid

    #172117

    Purpose

    Fluorescent reporter for CD63-positive exosome secretion

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    pHluorin_M153R in a small extracelluar loop of CD…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. myc-KIF5B

    Plasmid

    #127617

    Purpose

    N-terminal myc-tagged mouse KIF5B

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. 6xHis-SNAP-PI(3,4)P2 Probe

    Plasmid

    #211511

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    TAPP1 cPHx3
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. TEV Protease-mCherry

    Plasmid

    #243793

    Purpose

    Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TEV protease
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TEV-HRV-3C Proteases-mCherry

    Plasmid

    #243795

    Purpose

    Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TEV and HRV-3C proteases
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge