We narrowed to 9,400 results for: Coli

1. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-PLS-KP2_rpoA-U

   Plasmid

   #190956

   Purpose

   Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-PLS-KP6-A66S_rpoA-U

   Plasmid

   #190969

   Purpose

   Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:A66S

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PLS-KP3_rpoA-U

   Plasmid

   #190960

   Purpose

   Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-PLS-KP6-E67A_rpoA-U

   Plasmid

   #190973

   Purpose

   Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:E67A

   Promoter

   T7

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. hisSUMO-Gli1

   Plasmid

   #87225

   Purpose

   Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

   Depositor

   Gary Stormo

   Article

   Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

   Insert

   GLI1 (Gli1 Mouse)
   

   Use

   Tags

   hisSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a_UDP_Xase

   Plasmid

   #73158

   Purpose

   Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669

   Depositor

   Sandra Gabelli

   Article

   Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

   Insert

   pET24a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pRSETb-rsGreenF

   Plasmid

   #78181

   Purpose

   Expresses rsGreenF in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreenF
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTYF-1xGfaABC1D-tTA

    Plasmid

    #19977

    Depositor

    Sergey Kasparov

    Article

    Liu et al J Gene Med. 2008 May . 10(5):583-92.

    Insert

    GfaABC1D-tTA (GFAP E.coli, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-6xHis-ARL2

    Plasmid

    #67474

    Purpose

    Expression of hArl2 with N-term His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL2 (ARL2 Human)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pPPC027.306

    Plasmid

    #171149

    Purpose

    Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSW002-Pc-TorT(sp)

    Plasmid

    #111255

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT signal peptide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-ecDHFR-G51PEKN

    Plasmid

    #118582

    Purpose

    Bacterial expression of E.coli DHFR G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G51 mutated to P51; Glu-Lys-Asn residues inserted…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. His10-PS-SNAPf-9E (XSB526)

    Plasmid

    #78514

    Purpose

    Construct for expressing His10 - SNAPf -9E in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    SNAPf
    

    Use

    Tags

    His10 followed by a prescission protease claevage…

    Expression

    Bacterial

    Mutation

    9E added to the C-terminal of SNAPf

    Promoter

    Available Since

    June 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSPNprM-hp

    Plasmid

    #48120

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of NprM
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGS1011

    Plasmid

    #63783

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Strep-tag II followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSPVpr-hp

    Plasmid

    #48119

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of Vpr
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pANY1

    Plasmid

    #110949

    Purpose

    Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

    Insert

    ccd B cassette
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRSM15

    Plasmid

    #101640

    Purpose

    Encodes the fluorescent protein EG-4 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    EG-4
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-DEST42-ARL3

    Plasmid

    #67418

    Purpose

    Expression of hArl3 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pET21a - NDH

    Plasmid

    #89626

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD(P)-dependent oxidoreductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRSETb-rsGreen0.8

    Plasmid

    #78177

    Purpose

    Expresses rsGreen0.8 in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreen0.8
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28(a)-p2c-Cas9

    Plasmid

    #242759

    Purpose

    Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

    Depositor

    Jason Rasgon

    Article

    Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

    Insert

    P2C-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Sept. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHS1623

    Plasmid

    #239833

    Purpose

    WT OrufIscB E. coli expression for recombinant purification

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    OrufIscB
    

    Use

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET28-NFMtail

    Plasmid

    #232056

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET28a-OuIAD(E502Q)

    Plasmid

    #226973

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(E502Q)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    E502Q

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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