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beta4-nAChR (DPM negative control)

Plasmid

#86651

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (control)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-fugu SLC2A4 promoter

Plasmid

#65101

Purpose

Luciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.

Depositor

Josep Planas

Article

Marin-Juez et al PLoS One. 2013 Nov 18;8(11):e80628. doi:

Insert

SLC2A4 promoter

Use

Luciferase

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP

Plasmid

#114034

Purpose

Production of baculovirus with LacI-LAP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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SERA3-bio-His

Plasmid

#50815

Purpose

Expresses enzymatically monobiotinylated full length SERA3

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA3

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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SERA4-bio-His

Plasmid

#50816

Purpose

Expresses enzymatically monobiotinylated full length SERA4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA4

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF13_0125-bio-His

Plasmid

#50808

Purpose

Expresses enzymatically monobiotinylated full length PF13_0125

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF13_0125

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF14_0044-bio-His

Plasmid

#50809

Purpose

Expresses enzymatically monobiotinylated full length PF14_0044

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF14_0044

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PFA0135w-bio-His

Plasmid

#50810

Purpose

Expresses enzymatically monobiotinylated full length PFA0135w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0135w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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SERA2-bio-His

Plasmid

#50814

Purpose

Expresses enzymatically monobiotinylated full length SERA2

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA2

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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MSP3.5-bio-His

Plasmid

#50803

Purpose

Expresses enzymatically monobiotinylated full length MSP3.5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP3.5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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MSP8-bio-His

Plasmid

#50807

Purpose

Expresses enzymatically monobiotinylated full length MSP8 ectodomain

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSP8

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-pnr

Plasmid

#71503

Purpose

flySTARR-seq screening vector with pnr core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-rev

Plasmid

#81217

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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ARF17 pGreenII0229

Plasmid

#12070

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

ARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR4_TIAL1

Plasmid

#106089

Purpose

Gateway entry vector containing TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIAL1 (TIAL1 Human)

Use

Gateway entry vector

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJB128 (MatP-mEos2)

Plasmid

#72653

Purpose

Inducible expression of MatP-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

MatP-mEos2

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSiB3

Plasmid

#21958

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

BCL2L11 (Bcl2l11 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-PIM2mut1

Plasmid

#51849

Purpose

promoter region of PIM2 lacking the forkhead binding site #2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T-1-mMcl-1(S140A)

Plasmid

#25381

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(S140A) (Mcl1 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

changed serine 140 to alanine

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pSTARR-seq_fly-NipB

Plasmid

#71502

Purpose

flySTARR-seq screening vector with NipB core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhag-3'UTR

Plasmid

#61778

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pMB84 (Nf2 C-term)

Plasmid

#12169

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Nf2 C-terminal half (Nf2 Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

C-terminal half of Nf2 cDNA

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Flag3-pG-cNanog-pgk-hph

Plasmid

#74912

Purpose

Mammalian expression of Flag tagged cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Tags

3xFlag

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCLXSN-ING2

Plasmid

#13293

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pHU6_gRNA_Ch10-for1

Plasmid

#81210

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev1

Plasmid

#81208

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge