We narrowed to 11,244 results for: ENA
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Plasmid#51298Purposeexpression of the inactive mutant of mouse NRDc in mammalian cellsDepositorInsertNRDc E247A (Nrd1 Mouse)
TagsHIS and V5ExpressionMammalianMutationE247A, enzymatic inactive mutantPromoterCMVAvailable SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_ARP3B
Plasmid#111680PurposeMAC-tagged gene expressionDepositorInsertARP3B (ACTR3B Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos4b (mEosFP4b-V69T)
Plasmid#99226PurposeBacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).DepositorInsertpr-mEos4b
ExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-GFP-mSox2
Plasmid#206374PurposeExpresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.DepositorAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2
Plasmid#154149PurposeFor making TNKS-2 RNADepositorAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_AB
Plasmid#66004PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157839Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
ExpressionMammalianAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf42
Plasmid#12730PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf42
ExpressionMammalianAvailable SinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEXNKI-GST-3C-LIC
Plasmid#108710PurposeExpression of a GST-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsGST-3C protease cleavage siteExpressionBacterialPromotertacAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23D
Plasmid#91141PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantMutationD10A, H840AAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
Plasmid#136381PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-TV
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8CQ
Plasmid#52171PurposeEncodes module 8 with amino acids 12C-16Q for recognition of nt 1ADepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12C in module 8Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPKm-196
Plasmid#90511PurposepcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoterDepositorInsertPIF6-DBD
ExpressionMammalianAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf196
Plasmid#12884PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf196
ExpressionMammalianAvailable SinceDec. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2-3HA
Plasmid#169853PurposeMammalian Expression, SpyCas9 prime editor with 3HA-tagDepositorInsertSpCas9-H840A-M-MLV-3HA
Tags3XHA tag and bpSV40 NLSExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25G
Plasmid#91144PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
UseCRISPRExpressionPlantMutationD10A, H840AAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf117
Plasmid#12805PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf117
ExpressionMammalianAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only