4,182 results

1. mEGFP-pBAD

   Plasmid

   #54622

   Purpose

   Localization: Protein Expression Vector , Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis and mEGFP

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCIP-Flag-TfebAA

   Plasmid

   #79014

   Purpose

   For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (Tfeb Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S142A, S211A

   Promoter

   pCAGGs

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-Neo-Bam p53 R248W

   Plasmid

   #16437

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R248W (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R248W

   Available Since

   Aug. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C1(1-29)-miniTurbo-V5_pCDNA3

   Plasmid

   #107174

   Purpose

   expresses V5-tagged miniTurbo on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

   Plasmid

   #87306

   Purpose

   An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   Cre-dependent mouse codon-optimized Flp recombinase.
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG_smFP HA

   Plasmid

   #59759

   Purpose

   encodes multiple copies of an epitope tag HA

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _HA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-ABE7.10

   Plasmid

   #102919

   Purpose

   expresses ABE7.10 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.10
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. phOct4-EGFP

   Plasmid

   #38776

   Depositor

   Wei Cui

   Article

   Gerrard et al Stem Cells. 2005;23(1):124-33.

   Insert

   Oct-4 promoter (POU5F1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   human OCT4

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge