We narrowed to 11,935 results for: ARIA

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HA-USPL1-C236S-pcDNA3.1

Plasmid

#85763

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236S

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMS04 [myo-3p::bPGC::SL2::mCherry]

Plasmid

#168172

Purpose

Expression of bPGC in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

bPGC

Expression

Worm

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-plus-NLS-6xHis

Plasmid

#207381

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, Q695A, Q926A, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-SpCas9-HF1-NLS-6xHis

Plasmid

#207385

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LentiX_(loxPconDIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG3906

Plasmid

#246345

Purpose

loxPcon DIAL Reporter Lentivirus expressing mCherry-HRasG12V in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mCherry-GS-HRasG12V (HRAS Human)

Use

Lentiviral and Synthetic Biology

Mutation

G12V

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-mScarleti

Plasmid

#228526

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaR-SpCas9-NLS-6xHis

Plasmid

#207388

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A, amino acids 10…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A1003K

Plasmid

#207371

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1003K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A848K

Plasmid

#207369

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A848K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A661R

Plasmid

#207367

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-Q661R

Plasmid

#207366

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-Q661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, K526E, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-N515Y

Plasmid

#207364

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-N515Y

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTc253

Plasmid

#207464

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTc253 (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Mutation

Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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