We narrowed to 75,864 results for: Rest

1. pET3-ARL14-His6

   Plasmid

   #201878

   Purpose

   Used for bacterial expression of human protein ARL14

   Depositor

   Richard Kahn

   Insert

   ARL14 (ARL14 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET3-ARL8A-His6

   Plasmid

   #201872

   Purpose

   Used for bacterial expression of human protein ARL8A

   Depositor

   Richard Kahn

   Insert

   ARL8A (ARL8A Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET3-ARL3-His6

   Plasmid

   #201868

   Purpose

   Used for bacterial expression of human protein ARL3

   Depositor

   Richard Kahn

   Insert

   ARL3 (ARL3 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET3-ARL9-His6

   Plasmid

   #201874

   Purpose

   Used for bacterial expression of human protein ARL9

   Depositor

   Richard Kahn

   Insert

   ARL9 (ARL9 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKS_3HA_PAC

   Plasmid

   #122564

   Purpose

   Vector for endogenously tagging Giardia genes with a triple HA tag (Puromycin selection)

   Depositor

   Alexander Paredez, Zacheus Cande

   Article

   Gourguechon et al Eukaryot Cell. 2011 Jan;10(1):142-5. doi

   Type

   Empty backbone

   Use

   Tags

   hemagglutinin tag

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-Ex1-17-deltaP1P2-PTPN22

   Plasmid

   #195391

   Purpose

   Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tag

   Depositor

   Patrick Concannon

   Insert

   PTPN22 (PTPN22 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   PTPN22 mutant containing exon 1 to 17 and lacking…

   Promoter

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p3805

   Plasmid

   #198147

   Purpose

   Mammalian expression of LAMP1-miniSOG

   Depositor

   Michael Hensel

   Insert

   PCMV::LAMP1::miniSOG::mCherry (LAMP1 Human)
   

   Use

   Tags

   miniSOG-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3806

   Plasmid

   #198148

   Purpose

   Mammalian expression of LAMP1-miniSOG

   Depositor

   Michael Hensel

   Insert

   PCMV::LAMP1::tdminiSOG::mCherry (LAMP1 Human)
   

   Use

   Tags

   tdminiSOG-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-Ex1-17-deltaP2-PTPN22

   Plasmid

   #195395

   Purpose

   Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P2 domain) with FLAG tag

   Depositor

   Patrick Concannon

   Insert

   PTPN22 (PTPN22 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   PTPN22 mutant containing exon 1 to 17 and lacking…

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-Ex1-17-deltaP1-PTPN22

    Plasmid

    #195388

    Purpose

    Mammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 domain) with FLAG tag

    Depositor

    Patrick Concannon

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PTPN22 mutant containing exon 1 to 17 and lacking…

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

    Plasmid

    #194249

    Purpose

    Expresses 3 non-targeting miRNAs

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRNegative
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV/CBA::EGFP-P2A-tC3

    Plasmid

    #129476

    Purpose

    AAV2 transfer plasmid for truncated C3 with EGFP under control of the CBA promoter

    Depositor

    Robert Gross

    Insert

    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PVALB mA_BCN14.1.1C9_LC_Mouse Ig Kappa

    Plasmid

    #182889

    Purpose

    Mammalian expression of PVALB mouse mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid

    #182891

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a

    Plasmid

    #182892

    Purpose

    Mammalian expression of PVALB mouse mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PVALB mAb_BCN14.1.1A2_LC_Mouse Ig Kappa

    Plasmid

    #182893

    Purpose

    Mammalian expression of PVALB mouse mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CaMKII-Aion-ts-citrine-ER

    Plasmid

    #192576

    Purpose

    AAV-vector for optogenetic long-term silencing of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-Citrine-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mDlx-Aion-citrine

    Plasmid

    #192572

    Purpose

    AAV-vector for optogenetic long-term silencing of GABAergic neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    minimal Dlx

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid

    #192582

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid

    #192581

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hSyn-Aion-mKate2

    Plasmid

    #192574

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ipUSEPR-sg-Hs-KIF11

    Plasmid

    #188685

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgKIF11 (KIF11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ipUSEPR-sg-Hs-ACE2-A2

    Plasmid

    #188687

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ipUSEPR-sg-Hs-ACE2-A4

    Plasmid

    #188688

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p1X-UASTattB

    Plasmid

    #189861

    Purpose

    Fly cloning and transformation

    Depositor

    Jung Hwan Kim

    Article

    Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLIB_BSSHI_6xHisTEV-WIPI2d

    Plasmid

    #190863

    Purpose

    Plasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216

    Depositor

    Sascha Martens

    Insert

    WIPI2 (WIPI2 Human)
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    SNP C to G at position 4562 (Val)

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid

    #170788

    Purpose

    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Mortierella verticillate PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENN.AAV.SV40-si-MAPRE1

    Plasmid

    #187862

    Purpose

    a human siRNA-expressing plasmid targeting MAPRE1

    Depositor

    Yan Zeng

    Insert

    si-MAPRE1 (MAPRE1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCW57.1-GFP_mmu-miR-16-5p_sponge

    Plasmid

    #132624

    Purpose

    Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-16-5p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT2iD6Hoxa13bER4(#686)

    Plasmid

    #184101

    Purpose

    cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfHoxa13b-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEMHE-TNKS-2-M1054V

    Plasmid

    #138538

    Purpose

    For making TNKS-2-M1054V RNA

    Depositor

    Carmen Williams

    Insert

    Tankyrase 2 (TNKS2 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG

    Expression

    Mutation

    Changed methionine 1054 to valine

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pT2iD6Hoxa13aER4(#685)

    Plasmid

    #184100

    Purpose

    cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfHoxa13a-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid

    #183704

    Purpose

    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

    Depositor

    Jonathan Ploski

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

    Plasmid

    #183705

    Purpose

    AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

    Depositor

    Jonathan Ploski

    Insert

    hM3D(Gq)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQE31-6xHis-Countdown

    Plasmid

    #182299

    Purpose

    E. coli plasmid encoding Countdown with a hexahistidine tag

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    Countdown
    

    Use

    Tags

    histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQE31-6xHis-pHCountdown

    Plasmid

    #182300

    Purpose

    E. coli plasmid encoding pH-Countdown with a hexahistidine tag

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH Countdown
    

    Use

    Tags

    histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDZ2FE_Rev3_1873-2025

    Plasmid

    #105672

    Purpose

    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein

    Depositor

    Thanos Halazonetis

    Insert

    Rev3L (REV3L Human)
    

    Use

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid

    #167529

    Purpose

    To increase the length of the linker between Venus and Noxa-4E proteins

    Depositor

    David Andrews

    Insert

    PMA-induced protein 1 (PMAIP1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    C25E, L29E, F32E, L36E

    Promoter

    CMV

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p3xFLAG-CMV-fly Dicer

    Plasmid

    #171559

    Purpose

    Encodes fly Dicer protein

    Depositor

    Yan Zeng

    Insert

    Dcr-1(CG4792) (Dcr-1 Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRSET-QUEEN 7mu R126A

    Plasmid

    #129376

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu R126A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRSET-QUEEN 7mu R126I

    Plasmid

    #129377

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu R126I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRSET-QUEEN 7mu A81I

    Plasmid

    #129352

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu A81I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRSET-QUEEN 7mu D89A

    Plasmid

    #129354

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu D89A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRSET-QUEEN 7mu V90D

    Plasmid

    #129355

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu V90D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRSET-QUEEN 7mu A81E

    Plasmid

    #129356

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu A81E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRSET-QUEEN 7mu I88D

    Plasmid

    #129357

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu I88D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRSET-QUE7mu R122A

    Plasmid

    #129317

    Purpose

    mutant of QUEEN-7+D20

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-7mu R122A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pT2MPEF-NLS-QUE37C

    Plasmid

    #129326

    Purpose

    ATP measurement, nucleus, Tol2 transposon

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

49. pB80-empty

    Plasmid

    #174621

    Purpose

    Empty backbone

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pOpen-ECOligA

    Plasmid

    #165564

    Purpose

    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    E. coli DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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