We narrowed to 10,084 results for: Coli
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Plasmid#174664PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.DepositorInsertMS2 coat protein
TagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-Uricase
Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
ExpressionBacterialAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsZ
Plasmid#89656PurposePlasmid for titrating synthesis of FtsZ with doxycyclineDepositorInsertGTP-binding tubulin-like cell division protein
UseSynthetic BiologyAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(WT).J23115-gfp
Plasmid#197872PurposeArabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expressionDepositorInsertspart kilA/repL
gfp
ExpressionBacterialMutationcontains only the 24 bp of 3'-end of kilA ge…PromoterBba_J23115 and PBADAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorAvailable SinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'-t7- YFP
Plasmid#186564PurposepMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH67131
Plasmid#153223PurposeLevel 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminatorDepositorInsertNos promoter, NPTII, Nos terminator
UseSynthetic BiologyAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGK:TRL-EFS:iRFP670
Plasmid#68475PurposeLentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670DepositorInsertstTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670
UseLentiviral and LuciferaseExpressionMammalianPromoterEFS and pGKAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRCP
Plasmid#195862PurposeEncodes chromogenic protein (CP). CP inducible with rhamnose.DepositorInsertChromogenic Protein
ExpressionBacterialAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneTagsHis6-hPDIExpressionBacterialAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
ExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAP Z alpha 1 beta 1 CP
Plasmid#13451DepositorAvailable SinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
Ad-CBR (GFP/cAPC)
Plasmid#16576DepositorAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only