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pLBC0040

Plasmid

#172779

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0041

Plasmid

#172780

Purpose

Level 0 module containing CT_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPD355 VPR-FRB (NLS)

Plasmid

#138847

Purpose

Constitutive expression of VPR-FRB under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pKM377

Plasmid

#134210

Purpose

Baculovirus expression of CENP-HKM and His-CENP-I

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-H/K/M untagged + his-I

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Tags

mCherry

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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p6661 MSCV-IP N-HA only 16E6

Plasmid

#42603

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
YSBE 559 : pNAT_6xHA

Plasmid

#92087

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05

Plasmid

#67139

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and mCherry

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pXS-dTomato

Plasmid

#117387

Purpose

pGEN-mcs with a modular dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac FPH

Plasmid

#1925

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

polyhomeotic (ph-d, ph-p Fly)

Tags

Flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pPOTv7-hygro-BFP

Plasmid

#190260

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarlet

Plasmid

#179823

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOGG014

Plasmid

#113988

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 4

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Tags

His6-MBP

Expression

Bacterial

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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