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pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Use

Tags

FLAG

Expression

Mutation

Promoter

polyhedrin promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-N2-RIPK3 4KR

Plasmid

#78825

Purpose

To detect localization of RIPK3 4KR protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-K225insNM

Plasmid

#31974

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

inserted amino acids Asn-Met before Lys225

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 I136A, I139A, M142A

Plasmid

#74617

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 K627R

Plasmid

#78840

Purpose

To overexpress RIPK1 K627R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K627R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 K571R

Plasmid

#78838

Purpose

To overexpress RIPK1 K571R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIP-1 (RIPK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K571R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 K604R

Plasmid

#78839

Purpose

To overexpress RIPK1 K604R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K604R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1 K163R

Plasmid

#78836

Purpose

To overexpress RIPK1 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K163R

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NHA-PIWI(aa478-860)

Plasmid

#21541

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

contain only aa478-860

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pEGFPN1-Gm129

Plasmid

#53765

Purpose

mammalian expression of EGFP-Gm129

Depositor

Aziz Sancar

Article

Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

Insert

Gm129 (Ciart Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*

Promoter

CMV

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pGEMHE-MmCENP-W

Plasmid

#237433

Purpose

Expresses Mus musculus CENP-W; untagged; made for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mus musculus CENP-W (Cenpw Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENPT-GFP

Plasmid

#237426

Purpose

Expresses Mus musculus CENP-T tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mus musculus CENP-T (Cenpt Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F3A

Plasmid

#236437

Purpose

transient overexpression of E2F3A in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3A (E2F3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F3B

Plasmid

#236438

Purpose

transient overexpression of E2F3B in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3B (E2F3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE FLAG HA p100 (Ser707/711Ala)

Plasmid

#236469

Purpose

stable expression of p100 using virus infection

Depositor

Michele Pagano

Article

Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

Insert

p100 (NFKB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Ser707/711Ala

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xFLAG-2xSTREP TFDP1

Plasmid

#236444

Purpose

transient overexpression of TFDP1 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

TFDP1 (TFDP1 Human)

Use

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Cyclin F

Plasmid

#236466

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Use

Tags

Expression

Bacterial

Mutation

G120C

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Use

Tags

Expression

Bacterial

Mutation

G120C

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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tdiRFP-BFP reporter

Plasmid

#190184

Purpose

The cre-activatable fluorescence reporter

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

tdiRFP
TagBFP2

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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