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pFastBac FAK W266A

Plasmid

#186144

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Mutation

DNA mutation T796G;G797C Amino acid mutation W266A

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Lck

Plasmid

#186130

Purpose

For expression of recombinant Lck in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

Lck (Lck Mouse)

Tags

His6

Expression

Insect

Mutation

A1687T

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA.HA.HA.Mm.RAP1

Plasmid

#184646

Purpose

Mammalian expression of mouse Rap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

Plasmid

#149346

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Cas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbc

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-BbsI

Plasmid

#149347

Purpose

Mammalian expression vector for SpyCas9 and puromycin resistance

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

Plasmid

#149350

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0008

Plasmid

#117544

Purpose

Level 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7R8

Plasmid

#111041

Purpose

Plasmid expresses the talin2 rod domains R7 and R8

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7

Plasmid

#111039

Purpose

Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pVH004

Plasmid

#80401

Purpose

Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

CusR (cusR )
AraC (araC )
Anti-scaffold

Use

Synthetic Biology

Tags

C-terminal LZx domain

Expression

Bacterial

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pVH015

Plasmid

#80398

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold-ffGFP-3xFLAG
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal 3xFLAG tag and C-terminal LZx domain

Expression

Bacterial

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa

Plasmid

#78183

Purpose

Expresses pcDronpa in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3G08

Plasmid

#71044

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hrb98DE (Hrb98DE Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only