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pPB9

Plasmid

#210036

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
puro-T2A-eGFP

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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act-3p(long):rfp

Plasmid

#202330

Purpose

The act-3 promoter drives expression of TurboRFP in the pharynx, spermatheca, and body wall of C. elegans.

Depositor

Didier Stainier

Article

Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

Inserts

act-3p(long) promoter
TurboRFP

Use

Tags

Expression

Worm

Mutation

Promoter

This is a promoter sequence for C. elegans act-3 …

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh2CDS

Plasmid

#192841

Purpose

Plasmid to produce ISH probe for mMyh2 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 2 (Myh2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh2UTR

Plasmid

#192842

Purpose

Plasmid to produce ISH probe for mMyh2 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 2 (Myh2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh3CDS

Plasmid

#192843

Purpose

Plasmid to produce ISH probe for mMyh3 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 3 (Myh3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh3UTR

Plasmid

#192844

Purpose

Plasmid to produce ISH probe for mMyh3 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 3 (Myh3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh4CDS

Plasmid

#192845

Purpose

Plasmid to produce ISH probe for mMyh4 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 4 (Myh4 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh4UTR

Plasmid

#192846

Purpose

Plasmid to produce ISH probe for mMyh4 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 4 (Myh4 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh6CDS

Plasmid

#192847

Purpose

Plasmid to produce ISH probe for mMyh6 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 6 (Myh6 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh6UTR

Plasmid

#192848

Purpose

Plasmid to produce ISH probe for mMyh6 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 6 (Myh6 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh8UTR

Plasmid

#192852

Purpose

Plasmid to produce ISH probe for mMyh8 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 8 (Myh8 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-pSu9-HaloTag7-SNAP

Plasmid

#217319

Purpose

Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

Depositor

Noboru Mizushima

Article

Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

Insert

Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag

Use

Retroviral

Tags

HaloTag and SNAP

Expression

Mutation

Promoter

CMV

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCHC024 (∆SH)

Plasmid

#226202

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for soluble hydrogenase operon

Use

Synthetic Biology

Tags

Expression

Mutation

∆hoxFUYHWI ∆hypA2B2F2

Promoter

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC023 (∆MBH)

Plasmid

#226201

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for membrane bound hydrogenase operon

Use

Synthetic Biology

Tags

Expression

Mutation

∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

Promoter

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC022 (∆phcA)

Plasmid

#226200

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC004 (∆phaCAB)

Plasmid

#226198

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC027 (∆pemK)

Plasmid

#226204

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Upstream/downstream homology arms for addiction system toxinPemK.

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM94

Plasmid

#226686

Purpose

GGG-TitinV13PV15P-23-K-9-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Use

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM99

Plasmid

#226691

Purpose

TitinV15P-23-K-9-C-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Use

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM97

Plasmid

#226689

Purpose

GGG-Titinv15P-23-K-9-K-9-K-8-C-25

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Use

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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