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p-1459-adcy4-Luc

Plasmid

#28184

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

Adcy4 promoter (Adcy4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pJOG658

Plasmid

#105409

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

3xHA tag fused to Luciferase CDS

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_nucl

Plasmid

#113928

Purpose

Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_nucl

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-IVS-Syn21-GFP-P10

Plasmid

#104614

Purpose

Expresses GFP upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS3

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDMG8a

Plasmid

#33179

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

C27H6.9 3'UTR (C27H6.9 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG1a

Plasmid

#33158

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-fugu SLC2A4 promoter

Plasmid

#65101

Purpose

Luciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.

Depositor

Josep Planas

Article

Marin-Juez et al PLoS One. 2013 Nov 18;8(11):e80628. doi:

Insert

SLC2A4 promoter

Use

Luciferase

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
pJFRC81-3xVAS1-IVS-Syn21-GFP-P10

Plasmid

#104613

Purpose

Expresses GFP upon binding of TALE1 to VAS1

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS1

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mut

Plasmid

#48164

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Mut

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pAG249

Plasmid

#33202

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

WBP4 3'UTR (WBP4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR

Plasmid

#61778

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_3

Plasmid

#73527

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_4

Plasmid

#73528

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_2

Plasmid

#73526

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR

Plasmid

#61779

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
CJ40 (let-7 seed mutant perfect site)

Plasmid

#17773

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

let-7 seed mutant perfect site (let-7 Fly)

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pGL3-p107 2*

Plasmid

#32388

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

2nd E2F binding site mutated: GTC-->AAA

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry

Plasmid

#249563

Purpose

For detection of ER-mitochondrial contact

Depositor

Ginam Cho, Jeffrey Golden, Youngshin Lim

Article

Chen et al J Vis Exp. 2024 Oct 11;(212):10.3791/668

Insert

MitoRlucN-T2A-RlucCER

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Promoter

Chicken beta-actin promoter with CMV enhancer

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c26

Plasmid

#242875

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, A369P, 434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, A369P, 434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c15

Plasmid

#242873

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c10

Plasmid

#242872

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-empty

Plasmid

#239099

Purpose

pGL3 luciferase vector with no promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4 [luc2P/RBP-Jk-RE/Hygro] Vector

Plasmid

#236804

Purpose

Express RBP-Jk response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

RBP-Jk (RBPJ Human)

Use

Luciferase

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-PGK-TetOn-Puro_MeltITSN1-37-mch

Plasmid

#234068

Purpose

Encodes the Dbl homology and pleckstrin homology (DH-PH) domain of Intersectin1 controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltITSN1-37-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLS-CMV-mP-luc

Plasmid

#213506

Purpose

Positive control vector for luciferase lentivirus-based enhancer assay

Depositor

Nadav Ahituv

Article

Deng et al Science. 2024 May 24;384(6698):eadh0559.

Insert

Luciferase

Use

Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-dgat1b in situ probe

Plasmid

#232138

Purpose

zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-mogat2 in situ probe

Plasmid

#232139

Purpose

zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat2 (mogat2 Zebrafish)

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK83

Plasmid

#226793

Purpose

Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Insert

slr0214 (slr0214 Synechocystis sp. PCC 6803)

Expression

Bacterial

Promoter

Tetracycline resistance cassette promoter (consti…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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