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pSD138A

Plasmid

#218130

Purpose

Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

scP22

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-hGTB

Plasmid

#26196

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

F2A has 2A cleavage site deleted, producing htTA-…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-NG-nCas9

Plasmid

#163539

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMIR-His-Halo-Tev-PTEN

Plasmid

#58241

Purpose

expresses halo tagged PTEN protein

Depositor

Yimon Aye

Article

Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

Insert

phosphatase and tensin homolog (PTEN Human)

Tags

His-Halo-Tev

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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MRP1-CD4d3+4-bio

Plasmid

#73120

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding MRP1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

MRP1 (ABCC1 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-VRQR (pBM1173)

Plasmid

#140255

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_VRQR-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_Short-IRES-eGFP

Plasmid

#196935

Purpose

Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

short isoform, lack of signaling casacade

Promoter

TRE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

Plasmid

#81022

Purpose

Cancer-associated ASXL1 truncation mutant in retroviral vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

5’LTR

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-PSK1-beta

Plasmid

#197121

Purpose

Expression of MYC-tagged PSK1-beta in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

Insert

TAOK2 variant beta (aa1-1049) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Wild Type variant beta

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-TAPBPR-TM

Plasmid

#178645

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mIRFP670nuc-TEV-Xph20-ETWV

Plasmid

#133044

Purpose

Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

NLS, TEV protease + TEV cleavage site, and miRFP6…

Expression

Mammalian

Mutation

S63K

Promoter

CAG

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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ASC-CASP1 Octamer

Plasmid

#164032

Purpose

Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CASP1, ASC (CASP1, PYCARD Human)

Tags

His6-MBP-TEV

Expression

Bacterial

Mutation

W169G (ASC), G20K (CASP1)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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plenti-px330-CRBN-T2-pGK-Pur

Plasmid

#107383

Purpose

Mammalian expression CRISPR/Cas9

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

sgRNA targeting CRBN (CRBN Human)

Use

Lentiviral; Crispr

Expression

Mammalian

Promoter

U6

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJM185A

Plasmid

#218126

Purpose

Hybrid circuit displaying hsCRBN bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX020

Plasmid

#167146

Purpose

MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

fungal caffeoylpiruvate hydrolase, nnCPH

Use

Synthetic Biology

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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TGMP.shKras.1422

Plasmid

#59913

Purpose

Tet on shKras for conditional Kras knockdown

Depositor

Tyler Jacks

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

Kras (Kras Mouse)

Use

Retroviral

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

growth arrest specific 8 (Gas8 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

Plasmid

#163572

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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