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pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

Plasmid

#191362

Purpose

Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dBba2Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

Plasmid

#191361

Purpose

Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba3Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE‐NG‐loop

Plasmid

#187057

Purpose

ABEmax-SpCas9-NG-loop

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABE‐NG‐loop

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA008

Plasmid

#185754

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaEF1a (promoter)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA018

Plasmid

#185753

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaTip1;1 (promoter)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Emile Van Schaftingen, Liskin Swint-Kruse

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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NK72

Plasmid

#176608

Purpose

To test macrophage engulfment initiation. Lacks the FcyR chain.

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

CD86 (CD86 Human)

Use

Lentiviral

Tags

HA, SNAP-tag and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-mRuby3-iTrkA

Plasmid

#136510

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkA (Ntrk1 Avena sativa, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-mRuby3-iTrkB

Plasmid

#136511

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkB (aa 455-822)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII_F

Plasmid

#146298

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

Five non silent mutations A339T, V417M, R934G, D1…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179075

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-SCS2tmh

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179050

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179049

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179048

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179047

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179046

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179045

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-ATG8

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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