Skip to main content
Addgene
Showing: 4661 - 4680 of 4753 results

  2. pSMART-ala10

    Plasmid
    #87135
    Purpose
    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Article
    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.
    Inserts
    NADPH Alanine Dehydrogenase (ald Synthetic)
    alanine exporter (alaE Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D196A, L197R
    Promoter
    waaHp from E. coli as part of operon with ald* an…
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87135-plasmid-map-sequence-id-174451

    Enlarge

  7. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid
    #222878
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222878-plasmid-map-sequence-id-446610

    Enlarge

  8. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid
    #222884
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222884-plasmid-map-sequence-id-444499

    Enlarge

  9. L2116-FRB-GPA-114-RH-CyOFP1

    Plasmid
    #222883
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222883-plasmid-map-sequence-id-444497

    Enlarge

  10. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid
    #222875
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222875-plasmid-map-sequence-id-441838

    Enlarge

  11. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid
    #222891
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222891-plasmid-map-sequence-id-440208

    Enlarge

  12. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid
    #222886
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222886-plasmid-map-sequence-id-440205

    Enlarge

  13. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid
    #222888
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222888-plasmid-map-sequence-id-440203

    Enlarge

  14. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid
    #222887
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222887-plasmid-map-sequence-id-440200

    Enlarge

  15. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid
    #222890
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222890-plasmid-map-sequence-id-440190

    Enlarge

  16. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid
    #222889
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222889-plasmid-map-sequence-id-440187

    Enlarge

Showing: 4661 - 4680 of 4753 results