We narrowed to 7,101 results for: tev

1. p2CT-His-MBP-Lbu_C2c2_K310A

   Plasmid

   #91856

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K310A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K310A

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2CT-His-MBP-Lbu_C2c2_D1078A

   Plasmid

   #91860

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) D1078A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   D1078A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2CT-His-MBP-Lbu_C2c2_N314A

   Plasmid

   #91858

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) N314A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   N314A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lbu_C2c2_K1080A

   Plasmid

   #91862

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K1080A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1080A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubiquitin K6C/G76C

   Plasmid

   #86584

   Purpose

   Bacterial expression of Ubiquitin K6C/G76C

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K6C/G76C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Ubiquitin K48C/G76C

   Plasmid

   #86585

   Purpose

   Bacterial expression of Ubiquitin K48C/G76C

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K48C/G76C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Ubiquitin K63C/G76C

   Plasmid

   #86586

   Purpose

   Bacterial expression of Ubiquitin K63C/G76C

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K63C/G76C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. DMTF1

   Plasmid

   #36895

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DMTF1 (DMTF1 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains Myb-like 1 domain, residues 220-274

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Sgf73(1-106)/PET32a

   Plasmid

   #26962

   Depositor

   Cynthia Wolberger

   Article

   Samara et al Science. 2010 May 21. 328(5981):1025-9.

   Insert

   Sgf73(1-106) (SGF73 Budding Yeast)
   

   Use

   Tags

   His6 and Trx

   Expression

   Bacterial

   Mutation

   with TEV site to cleave the N-terminal his+Trx tag

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLIC.B4.TM1717

    Plasmid

    #11532

    Depositor

    Sung-Hou Kim

    Article

    Shin et al Proc Natl Acad Sci U S A. 2004 Sep 7. 10

    Insert

    YjeQ GDP binding protein (TM1717 T. maritima)
    

    Use

    Tags

    HisTag-MBP, TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

11. L284F Cus2p

    Plasmid

    #73059

    Purpose

    S. cerevisiae Cus2p gene with L284F Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Use

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Leucine 284 to Phenylalanine

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. D282N Cus2p

    Plasmid

    #73060

    Purpose

    S. cerevisiae Cus2p gene with D282N Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Use

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Asp 282 to Asn

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSP1D1

    Plasmid

    #20061

    Purpose

    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    deletion mutant (1-11) of MSP1; His-tag on N-term…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNIC28-Bsa4

    Plasmid

    #26103

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH-MBP-CbAgo

    Plasmid

    #127707

    Purpose

    pET His6 MBP TEV CbAgo

    Depositor

    John van der Oost

    Article

    Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

    Insert

    CbAgo
    

    Use

    Tags

    MPB

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNH-TrxT

    Plasmid

    #26106

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis, TEV cleavage site, and TrxA

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p2CT-His-MBP-Lwa_Cas13a_WT

    Plasmid

    #91865

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LwaCas13a
    

    Use

    CRISPR

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge